Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells.
Lipoteichoic Acid of Probiotic Lactobacillus plantarum Attenuates Poly I:C-Induced IL-8 Production in Porcine Intestinal Epithelial Cells.
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DOI：
10.3389/fmicb.2017.01827
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发表时间：
2017
影响因子：
5.2
通讯作者：
Han SH
中科院分区：
文献类型：
--
作者：
Kim KW;Kang SS;Woo SJ;Park OJ;Ahn KB;Song KD;Lee HK;Yun CH;Han SH
家畜饲料补充剂中的益生菌被认为是增强胃肠道免疫力的抗生素的替代品。虽然细菌细胞壁组分已被提出与益生菌功能相关，但很少有证据表明它们负责家畜中的益生菌功能。本研究证明植物乳杆菌的脂磷壁酸（LTA）在猪小肠上皮细胞系IPEC-J2中具有抗炎作用。病毒双链RNA的合成类似物poly I：C在IPEC-J2细胞中在mRNA和蛋白水平上剂量依赖性地诱导IL-8的产生。LTA，而不是脂蛋白或肽聚糖从L。plantarum，专门抑制聚I：C诱导的IL-8的产生。与其他益生乳酸菌菌株（包括L.德尔布鲁基湖sakei和L. LTA对poly I：C诱导的IL-8产生有较强的抑制作用。脱丙氨酰化或脱酰化的Lp.LTA不抑制聚I：C诱导的IL-8产生，表明Lp.LTA结构中的D-丙氨酸和脂质部分负责抑制。LTA还可抑制ERK和p38激酶的磷酸化以及NF-κB的活化，从而降低IL-8的产生。综上所述，这些结果表明，Lp.LTA作为一种效应分子，抑制病毒病原体诱导的猪肠上皮细胞的炎症反应。
Probiotics in livestock feed supplements are considered a replacement for antibiotics that enhance gastrointestinal immunity. Although bacterial cell wall components have been proposed to be associated with probiotic function, little evidence demonstrates that they are responsible for probiotic functions in livestock. The present study demonstrated that lipoteichoic acid (LTA) of Lactobacillus plantarum (Lp.LTA) confers anti-inflammatory responses in porcine intestinal epithelial cell line, IPEC-J2. A synthetic analog of viral double-stranded RNA, poly I:C, dose-dependently induced IL-8 production at the mRNA and protein levels in IPEC-J2 cells. Lp.LTA, but not lipoprotein or peptidoglycan from L. plantarum, exclusively suppressed poly I:C-induced IL-8 production. Compared with LTAs from other probiotic Lactobacillus strains including L. delbrueckii, L. sakei, and L. rhamnosus GG, Lp.LTA had higher potential to suppress poly I:C-induced IL-8 production. Dealanylated or deacylated Lp.LTA did not suppress poly I:C-induced IL-8 production, suggesting that D-alanine and lipid moieties in the Lp.LTA structure were responsible for the inhibition. Furthermore, Lp.LTA attenuated the phosphorylation of ERK and p38 kinase as well as the activation of NF-κB, resulting in decreased IL-8 production. Taken together, these results suggest that Lp.LTA acts as an effector molecule to inhibit viral pathogen-induced inflammatory responses in porcine intestinal epithelial cells.
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影响因子：
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