Coupling supported lipid bilayer electrophoresis with matrix-assisted laser desorption/ionization-mass spectrometry imaging.
Coupling supported lipid bilayer electrophoresis with matrix-assisted laser desorption/ionization-mass spectrometry imaging.
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DOI：
10.1021/ac4008804
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发表时间：
2013-06-18
影响因子：
7.4
通讯作者：
Cremer, Paul S.
中科院分区：
文献类型：
--
作者：
Pace, Hudson P.;Sherrod, Stacy D.;Monson, Christopher F.;Russell, David H.;Cremer, Paul S.
在这里，我们描述了一个新的分析平台，利用异质支撑脂质双层（SLB）电泳和基质辅助激光解吸/电离质谱（MALDI-MS）成像的进展。该平台允许带电和中性脂质膜成分的分离和可视化，而无需外部标记。使用含有单唾液酸神经节苷脂GM 1、二唾液酸神经节苷脂GD 1b、POPC以及德克萨斯红-DHPE的邻位和帕拉异构体的囊泡产生异质SLB。然后将这些组分经色谱分离成五个分辨带。这代表了迄今为止通过SLB电泳进行的最复杂的分离。将SLB样品在液体乙烷中快速冷冻并在真空下干燥，然后用MALDI-MS成像。荧光显微镜用于确认德克萨斯红标记的脂质的位置，其与MALDI-MS成像结果一致。这些结果清楚地证明了该平台分离和鉴定SLB内未标记膜组分的能力。
Herein we describe a new analytical platform utilizing advances in heterogeneous supported lipid bilayer (SLB) electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging. This platform allowed for the separation and visualization of both charged and neutral lipid membrane components without the need for extrinsic labels. A heterogeneous SLB was created using vesicles containing monosialoganglioside GM1, disialoganglioside GD1b, POPC, as well as the ortho- and para- isomers of Texas Red-DHPE. These components were then separated electrophoretically into five resolved bands. This represents the most complex separation by SLB electrophoresis performed to date. The SLB samples were flash frozen in liquid ethane and dried under vacuum before imaging with MALDI-MS. Fluorescence microscopy was employed to confirm the position of the Texas Red labeled lipids, which agreed well with the MALDI-MS imaging results. These results clearly demonstrate this platform’s ability to isolate and identify non-labeled membrane components within an SLB.
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影响因子：
7.4
作者：
Liu, Chunming;Monson, Christopher F.;Yang, Tinglu;Pace, Hudson;Cremer, Paul S.
通讯作者：
Cremer, Paul S.
影响因子：
56.9
作者：
Kraft, Mary L.;Weber, Peter K.;Boxer, Steven G.
通讯作者：
Boxer, Steven G.
影响因子：
56.9
作者：
Sackmann, E
通讯作者：
Sackmann, E
影响因子：
6.7
作者：
Heeren, R. M. A.;McDonnell, L. A.;Piersma, S. R.
通讯作者：
Piersma, S. R.
影响因子：
3.3
作者：
Cremer, PS;Boxer, SG
通讯作者：
Boxer, SG