Ionic Liquid-Based Ultrasonic-Assisted Extraction of Secoisolariciresinol Diglucoside from Flaxseed (Linum usitatissimum L.) with Further Purification by an Aqueous Two-Phase System.
Ionic Liquid-Based Ultrasonic-Assisted Extraction of Secoisolariciresinol Diglucoside from Flaxseed (Linum usitatissimum L.) with Further Purification by an Aqueous Two-Phase System.
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基于离子液体的超声辅助从亚麻籽 (Linum usitatissimum L.) 中提取开环异落叶松树脂醇二葡萄糖苷并通过水性两相系统进一步纯化
DOI：
10.3390/molecules201017929
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发表时间：
2015-09-30
期刊：
影响因子：
--
通讯作者：
Li FF
中科院分区：
文献类型：
--
作者：
Tan ZJ;Wang CY;Yang ZZ;Yi YJ;Wang HY;Zhou WL;Li FF
采用离子液体超声辅助萃取（IL-UAE）和离子液体双水相萃取（IL-ATPS）两步法从亚麻籽中提取并纯化开环异落叶松树脂酚二葡萄糖苷（SDG）。在IL-UAE步骤中，研究了几种IL作为提取剂，以确定提供最佳提取率的IL。采用响应面法对IL浓度、超声时间、液料比等提取条件进行优化。在IL-ATPS步骤中，通过向IL提取物中加入亲液盐形成的ATPS用于SDG的进一步分离和纯化。最有影响力的参数（类型和浓度的盐，温度和pH值）进行了研究，以获得最佳的提取效率。在22 °C、pH 11.0、45.86%（w/w）IL和8.27%（w/w）Na 2SO 4的最佳提取条件下，最大提取率为93.35%。因此，IL-UAE和IL-ATPS的组合构成了用于SDG的提取和纯化的简单有效的方法。这一过程也被认为是非常有用的提取和纯化的生物活性化合物从其他重要的药用植物。
In this work, a two-step extraction methodology of ionic liquid-based ultrasonic-assisted extraction (IL-UAE) and ionic liquid-based aqueous two-phase system (IL-ATPS) was developed for the extraction and purification of secoisolariciresinol diglucoside (SDG) from flaxseed. In the IL-UAE step, several kinds of ILs were investigated as the extractants, to identify the IL that affords the optimum extraction yield. The extraction conditions such as IL concentration, ultrasonic irradiation time, and liquid–solid ratio were optimized using response surface methodology (RSM). In the IL-ATPS step, ATPS formed by adding kosmotropic salts to the IL extract was used for further separation and purification of SDG. The most influential parameters (type and concentration of salt, temperature, and pH) were investigated to obtain the optimum extraction efficiency. The maximum extraction efficiency was 93.35% under the optimal conditions of 45.86% (w/w) IL and 8.27% (w/w) Na2SO4 at 22 °C and pH 11.0. Thus, the combination of IL-UAE and IL-ATPS makes up a simple and effective methodology for the extraction and purification of SDG. This process is also expected to be highly useful for the extraction and purification of bioactive compounds from other important medicinal plants.
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影响因子：
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