Wolbachia variants induce differential protection to viruses in Drosophila melanogaster: a phenotypic and phylogenomic analysis.
Wolbachia variants induce differential protection to viruses in Drosophila melanogaster: a phenotypic and phylogenomic analysis.
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DOI：
10.1371/journal.pgen.1003896
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发表时间：
2013
期刊：
影响因子：
4.5
通讯作者：
Teixeira L
中科院分区：
文献类型：
--
作者：
Chrostek E;Marialva MS;Esteves SS;Weinert LA;Martinez J;Jiggins FM;Teixeira L
沃尔巴克氏菌是一种胞内细菌共生体，能够保护各种昆虫宿主免受病毒感染。这种三方相互作用最初是在携带wMel的果蝇身上描述的，wMel是果蝇的天然沃尔巴克氏菌株。WMel已被证明在遗传上是多态的，而且最近在自然群体中的变异频率发生了变化。我们比较了不同wmel变异体提供的抗病毒保护，它们的滴度和对宿主寿命的影响，在一个遗传相同的黑腹果蝇宿主中。表型将变异体分成两组-wMelCS-like和wMel-like。WMelCS样变异体对果蝇C病毒和鸡舍病毒有更强的保护作用，滴度更高，通常会缩短宿主的寿命。我们对这些沃尔巴克氏菌的基因组进行了测序和组装，结果表明这两个表型群是两个单系群。我们还分析了一种剧毒和过度复制的变种wMelPop，它比密切相关的wMelCS更能保护黑腹葡萄球菌。我们发现，基因组的∼21kb区域编码8个基因，在wMelPop中被扩增7倍，这可能是其表型的原因。我们的结果表明，保护性较强的wMelCS样变异体，有时是有成本的，被保护性较弱但更良性的wMel样变异体所取代。这导致了最近全球自然种群中黑腹果蝇对病毒的抵抗力下降。我们的工作有助于了解wMel的自然变异及其进化动力学，并为Wolbachia在节肢动物传播疾病控制中的应用提供信息。沃尔巴克氏菌是一种细菌共生体，可以感染许多节肢动物，并可以保护昆虫免受病毒感染。在这里，我们展示了黑腹果蝇沃尔巴克氏菌的不同变体(WMel)具有表型异质性：它们提供的保护水平不同，它们在宿主体内达到的滴度也不同。滴度较高的沃尔巴克氏菌具有较高的抗病毒保护作用，但也会对宿主造成成本。根据观察到的表型，我们将wMel变异体分为两组，并证明这种划分反映在它们的系统发育中。此外，我们发现了两个几乎相同的wMEL变体wMelPop和wMelCS之间的遗传差异，这可能解释了为什么一个是高致病性的，而另一个会产生良性感染。我们的研究有助于解释不同wMel变异体在野生果蝇种群中的流行情况，并揭示形成这种变异体的因素。特别是，最近一些wmel变体的替换导致了抗病毒抵抗力的下降，并可能降低了宿主共生体的成本。最后，我们的工作有助于了解wmel与其自然宿主的相互作用，并为Wolbachia在控制昆虫传播疾病方面的应用提供信息。
Wolbachia are intracellular bacterial symbionts that are able to protect various insect hosts from viral infections. This tripartite interaction was initially described in Drosophila melanogaster carrying wMel, its natural Wolbachia strain. wMel has been shown to be genetically polymorphic and there has been a recent change in variant frequencies in natural populations. We have compared the antiviral protection conferred by different wMel variants, their titres and influence on host longevity, in a genetically identical D. melanogaster host. The phenotypes cluster the variants into two groups — wMelCS-like and wMel-like. wMelCS-like variants give stronger protection against Drosophila C virus and Flock House virus, reach higher titres and often shorten the host lifespan. We have sequenced and assembled the genomes of these Wolbachia, and shown that the two phenotypic groups are two monophyletic groups. We have also analysed a virulent and over-replicating variant, wMelPop, which protects D. melanogaster even better than the closely related wMelCS. We have found that a ∼21 kb region of the genome, encoding eight genes, is amplified seven times in wMelPop and may be the cause of its phenotypes. Our results indicate that the more protective wMelCS-like variants, which sometimes have a cost, were replaced by the less protective but more benign wMel-like variants. This has resulted in a recent reduction in virus resistance in D. melanogaster in natural populations worldwide. Our work helps to understand the natural variation in wMel and its evolutionary dynamics, and inform the use of Wolbachia in arthropod-borne disease control. Wolbachia are bacterial symbionts that infect many arthropods and can protect insects from viral infection. Here we show that different variants of Wolbachia from Drosophila melanogaster (wMel) are phenotypically heterogeneous: they differ in the level of protection they confer and the titres they reach in their host. The Wolbachia with higher titres have higher antiviral protection but can also exert a cost on their host. Based on the observed phenotypes, we divided the wMel variants into two groups and demonstrated that the division is reflected in their phylogeny. Moreover, we discovered the genetic difference between two otherwise almost identical wMel variants, wMelPop and wMelCS, that may explain why one is highly pathogenic while the other produces benign infections. Our study helps to explain the prevalence of the different wMel variants in wild Drosophila populations and sheds light on the factors shaping it. In particular, the recent replacement of some wMel variants caused a decrease in anti-viral resistance and probably reduced the cost of the symbiont for the host. Finally, our work helps to understand the interaction of wMel with its natural host and inform Wolbachia use in the control of diseases transmitted by insects.
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古老的内共生体内插入序列的强烈替代活性。
影响因子：
10.7
作者：
Cordaux, Richard;Pichon, Samuel;Ling, Alison;Perez, Philippe;Delaunay, Carine;Vavre, Fabrice;Bouchon, Didier;Greve, Pierre
通讯作者：
Greve, Pierre
DOI：
10.1126/science.1197761
发表时间：
2011-02-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Colbourne JK;Pfrender ME;Gilbert D;Thomas WK;Tucker A;Oakley TH;Tokishita S;Aerts A;Arnold GJ;Basu MK;Bauer DJ;Cáceres CE;Carmel L;Casola C;Choi JH;Detter JC;Dong Q;Dusheyko S;Eads BD;Fröhlich T;Geiler-Samerotte KA;Gerlach D;Hatcher P;Jogdeo S;Krijgsveld J;Kriventseva EV;Kültz D;Laforsch C;Lindquist E;Lopez J;Manak JR;Muller J;Pangilinan J;Patwardhan RP;Pitluck S;Pritham EJ;Rechtsteiner A;Rho M;Rogozin IB;Sakarya O;Salamov A;Schaack S;Shapiro H;Shiga Y;Skalitzky C;Smith Z;Souvorov A;Sung W;Tang Z;Tsuchiya D;Tu H;Vos H;Wang M;Wolf YI;Yamagata H;Yamada T;Ye Y;Shaw JR;Andrews J;Crease TJ;Tang H;Lucas SM;Robertson HM;Bork P;Koonin EV;Zdobnov EM;Grigoriev IV;Lynch M;Boore JL
通讯作者：
Boore JL
影响因子：
56.9
作者：
AXELROD, R;HAMILTON, WD
通讯作者：
HAMILTON, WD
影响因子：
9.8
作者：
Ayres JS;Schneider DS
通讯作者：
Schneider DS
影响因子：
3.7
作者：
Berry B;Deddouche S;Kirschner D;Imler JL;Antoniewski C
通讯作者：
Antoniewski C