Cellular interaction and toxicity depend on physicochemical properties and surface modification of redox-active nanomaterials.
Cellular interaction and toxicity depend on physicochemical properties and surface modification of redox-active nanomaterials.
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DOI：
10.1021/nn305872d
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发表时间：
2013-06-25
期刊：
影响因子：
17.1
通讯作者：
Self, William T.
中科院分区：
文献类型：
--
作者：
Dowding, Janet M.;Das, Soumen;Kumar, Amit;Dosani, Talib;McCormack, Rameech;Gupta, Ankur;Sayle, Thi X. T.;Sayle, Dean C.;von Kalm, Laurence;Seal, Sudipta;Self, William T.
关键词：
由于CeO2 NPs （CNPs）的治疗潜力，近年来对其化学和生物学特性的研究得到了扩展，并且用于合成这些材料的方法多种多样。此外，关于CNP的毒性存在相互矛盾的报道。为了帮助解决这些差异，我们必须首先确定不同方法制备的CeO2 NPs在物理化学和催化性能上是否相似或不同。在本文中，我们通过湿法合成了几种形式的CNPs，使用相同的前体，但使用不同的氧化剂/还原剂H2O2 (CNP1), NH4OH （CNP2）或六亚甲基四胺（HMT-CNP1）。对这些纳米粒子的理化性质进行了广泛的研究，发现制备方法不同，纳米粒子的理化性质也不同。与CNP1和CNP2不同，HMT-CNP1很容易进入内皮细胞，并且可以使用光学显微镜观察它们的聚集。暴露于HMT-CNP1也降低了细胞活力（MTT），浓度比CNP1或CNP2低10倍。令人惊讶的是，暴露于HMT-CNP1导致ATP水平大幅下降。机制研究表明HMT-CNP1表现出大量的atp酶（磷酸酶）活性。虽然CNP2也表现出atp酶活性，但CNP1缺乏atp酶活性。不同制备的CeO2 NPs的催化（atp酶）活性的差异可能是由于其形态和氧萃取能量的差异。这些结果表明，HMT-CNP1摄取和atp酶活性增加的结合可能是这种CNPs制备毒性的生物机制的基础，并且可能表明在合成用于生物医学应用的CNPs时应考虑atp酶活性。
The study of the chemical and biological properties of CeO2 NPs (CNPs) has expanded recently due to its therapeutic potential, and the methods used to synthesize these materials are diverse. Moreover, conflicting reports exists regarding the toxicity of CNP. To help resolve these discrepancies, we must first determine whether CeO2 NPs made by different methods are similar or different in their physiochemical and catalytic properties. In this paper, we have synthesized several forms of CNPs using identical precursors through a wet chemical process but using different oxidizer/reducer H2O2 (CNP1), NH4OH (CNP2) or hexamethylenetetramine (HMT-CNP1). Physiochemical properties of these CeO2 NPs were extensively studied and found to be different depending on the preparation methods. Unlike CNP1 and CNP2, HMT-CNP1 were readily taken into endothelial cells and their aggregation can be visualized using light microscopy. Exposure to HMT-CNP1 also reduced cell viability (MTT) at a 10-fold lower concentration than CNP1 or CNP2. Surprisingly, exposure to HMT-CNP1 led to substantial decreases in the ATP levels. Mechanistic studies revealed that HMT-CNP1 exhibited substantial ATPase (phosphatase) activity. Though CNP2 also exhibits ATPase activity, CNP1 lacked ATPase activity. The difference in catalytic (ATPase) activity of different CeO2 NPs preparation may be due to differences in their morphology and oxygen extraction energy. These results suggest the combination of increased uptake and ATPase activity of HMT-CNP1 may underlie the biomechanism of the toxicity of this preparation of CNPs, and may suggest ATPase activity should be considered when synthesizing CNPs for use in biomedical applications.
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影响因子：
14
作者：
Das S;Singh S;Dowding JM;Oommen S;Kumar A;Sayle TX;Saraf S;Patra CR;Vlahakis NE;Sayle DC;Self WT;Seal S
通讯作者：
Seal S
DOI：
10.1016/j.nano.2010.05.004
发表时间：
2010-12-01
影响因子：
5.4
作者：
Kuchma, Melissa Hirsch;Komanski, Christopher B.;Baker, Cheryl H.
通讯作者：
Baker, Cheryl H.
影响因子：
3.7
作者：
Karakoti, A. S.;Kuchibhatla, Satyanarayana V. N. T.;Seal, S.
通讯作者：
Seal, S.
影响因子：
15
作者：
Karakoti, Ajay S.;Singh, Sanjay;Kumar, Amit;Malinska, Maura;Kuchibhatla, Satyanarayana V. N. T.;Wozniak, Krzysztof;Self, William T.;Seal, Sudipta
通讯作者：
Seal, Sudipta
影响因子：
4.9
作者：
Dowding, Janet M.;Dosani, Talib;Self, William T.
通讯作者：
Self, William T.