At-Home Saliva Sampling in Healthy Adults Using CandyCollect, a Lollipop-Inspired Device.
At-Home Saliva Sampling in Healthy Adults Using CandyCollect, a Lollipop-Inspired Device.
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DOI：
10.1021/acs.analchem.3c00462
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发表时间：
2023-07-11
影响因子：
7.4
通讯作者：
Theberge, Ashleigh B.
中科院分区：
文献类型：
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作者：
Tu, Wan-chen;McManamen, Anika M.;Su, Xiaojing;Jeacopello, Ingrid;Takezawa, Meg G.;Hieber, Damielle L.;Hassan, Grant W.;Lee, Ulri N.;Anana, Eden V.;Locknane, Mason P.;Stephenson, Molly W.;Shinkawa, Victoria A. M.;Wald, Ellen R.;DeMuri, Gregory P.;Adams, Karen N.;Berthier, Erwin;Thongpang, Sanitta;Theberge, Ashleigh B.
呼吸道感染在儿童中很常见，因此需要用户友好的收集方法。在这里，我们使用 CandyCollect 设备进行了首次人类受试者研究，这是一种受棒棒糖启发的唾液收集设备。我们展示了 CandyCollect 设备可用于使用变形链球菌和金黄色葡萄球菌作为概念验证共生细菌来收集健康成年人的唾液细菌。我们招募了健康成年人参加一项全国性（美国）远程研究，其中向参与者发送了包含 CandyCollect 设备和传统市售口腔拭子和唾液管的研究包。参与者在家中自行取样，完成可用性和用户偏好调查，并将样本邮寄回我们的实验室进行 qPCR 分析。我们的结果表明，对于通过一种或两种市售方法（口腔拭子和/或唾液管）检测到特定细菌（变形链球菌或金黄色葡萄球菌）的参与者，CandyCollect 设备与阳性结果的一致性为 100%（n=14 名参与者）。此外，CandyCollect 设备被 26 名接受调查的参与者（结合两个注册组的调查结果）评为三种抽样方法中最偏好的抽样方法。我们还展示了CandyCollect设备在室温下的保质期长达1年，这个储存期限方便诊所或患者保留CandyCollect设备并随时使用。综上所述，我们已经证明 CandyCollect 是一种用户友好的唾液采集工具，有可能被纳入诊所和远程医疗的诊断分析中。
Respiratory infections are common in children, and there is a need for user-friendly collection methods. Here, we performed the first human subjects study using the CandyCollect device, a lollipop inspired saliva collection device. We showed the CandyCollect device can be used to collect salivary bacteria from healthy adults using Streptococcus mutans and Staphylococcus aureus as proof-of-concept commensal bacteria. We enrolled healthy adults in a nationwide (USA) remote study in which participants were sent study packages containing CandyCollect devices and traditional commercially available oral swabs and spit tubes. Participants sampled themselves at home, completed usability and user preference surveys, and mailed the samples back to our laboratory for analysis by qPCR. Our results showed that for participants in which a given bacterium (S. mutans or S. aureus) was detected in one or both of the commercially available methods (oral swab and/or spit tubes), CandyCollect devices had a 100% concordance with the positive result (n=14 participants). Furthermore, the CandyCollect device was ranked the highest preference sampling method among the three sampling methods by 26 participants surveyed (combining survey results across two enrollment groups). We also showed that the CandyCollect device has a shelf life of up to 1 year at room temperature, a storage period that is convenient for clinics or patients to keep the CandyCollect device and use it any time. Taken together, we have demonstrated that the CandyCollect is a user-friendly saliva collection tool that has the potential to be incorporated into diagnostic assays in clinic visits and telemedicine.
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影响因子：
14.9
作者：
Koblitz, Julia;Halama, Philipp;Spring, Stefan;Thiel, Vera;Baschien, Christiane;Hahnke, Richard L.;Pester, Michael;Overmann, Joerg;Reimer, Lorenz Christian
通讯作者：
Reimer, Lorenz Christian
影响因子：
--
作者：
Pannu P;Gambhir R;Sujlana A
通讯作者：
Sujlana A
影响因子：
1.6
作者：
DeMuri, Gregory;Wald, Ellen R.
通讯作者：
Wald, Ellen R.
DOI：
10.3390/v14091931
发表时间：
2022-08-30
期刊：
Viruses
影响因子：
--
作者：
De Meyer J;Goris H;Mortelé O;Spiessens A;Hans G;Jansens H;Goossens H;Matheeussen V;Vandamme S
通讯作者：
Vandamme S
影响因子：
6.4
作者：
Martín R;Miquel S;Ulmer J;Kechaou N;Langella P;Bermúdez-Humarán LG
通讯作者：
Bermúdez-Humarán LG