Ultraviolet radiation-induced degradation of dermal extracellular matrix and protection by green tea catechins: a randomized controlled trial.
Ultraviolet radiation-induced degradation of dermal extracellular matrix and protection by green tea catechins: a randomized controlled trial.
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紫外线诱导真皮细胞外基质降解及绿茶儿茶素的保护作用：一项随机对照试验。
DOI：
10.1111/ced.15179
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发表时间：
2022-07
影响因子：
4.1
通讯作者：
中科院分区：
文献类型：
--
作者：
真皮细胞外基质（ECM）的缺失和重塑是光损伤人类皮肤的关键特征。绿茶儿茶素（GTCs）因其抗炎和化学预防特性已被研究，但关于GTCs对紫外线辐射（UVR）诱导的真皮ECM变化的影响的数据尚缺乏。
在一项双盲随机对照试验中，研究炎症剂量的模拟太阳紫外线辐射对人类真皮ECM的影响以及GTCs的潜在保护作用。
共有50名年龄在18 - 65岁的健康白人（菲茨帕特里克皮肤类型I - II）成年人被随机分配，一组每天两次服用540毫克GTCs加50毫克维生素C，另一组服用安慰剂，持续12周。通过组织学和免疫组织化学在基线时评估所有参与者中3×最小红斑剂量的模拟太阳紫外线辐射对真皮胶原蛋白和弹性纤维网络的影响。在补充后比较两组之间GTC补充对紫外线辐射诱导效应的影响。
紫外线辐射暴露皮肤中胶原蛋白和弹性纤维覆盖的乳头层真皮面积显著低于（P < 0.001）未暴露皮肤。紫外线辐射暴露皮肤中富含原纤维蛋白的微纤维（P = 0.02）、纤维连接蛋白 - 2（P < 0.001）和纤维连接蛋白 - 5（P < 0.001）水平显著低于未暴露皮肤，而紫外线辐射暴露皮肤中前胶原蛋白 - 1沉积显著更高（P = 0.01）。在补充GTC后，活性组中紫外线辐射诱导的纤维连接蛋白 - 5变化被消除，但安慰剂组没有，两组在其他成分上没有差异。
急性紫外线辐射导致人类真皮胶原蛋白和弹性纤维网络发生显著变化，而口服GTCs对纤维连接蛋白 - 5提供了特定的紫外线辐射保护。未来的研究可以探索GTCs对人类皮肤反复亚红斑量紫外线辐射暴露影响的作用。
人类皮肤长期暴露于阳光中的紫外线辐射（UVR）会导致真皮细胞外基质成分的缺失和重塑，急性紫外线辐射暴露也可能导致光损伤。单次晒伤剂量的模拟太阳紫外线辐射导致整体胶原蛋白和弹性纤维网络，特别是富含原纤维蛋白的微纤维、纤维连接蛋白 - 2和纤维连接蛋白 - 5显著减少。口服绿茶儿茶素补充剂消除了紫外线辐射诱导的纤维连接蛋白 - 5减少，但对其他真皮基质参数没有显著影响。
Loss and remodelling of the dermal extracellular matrix (ECM) are key features of photodamaged human skin. Green tea catechins (GTCs) have been explored for their anti‐inflammatory and chemopreventive properties, but data on the impact of GTCs on ultraviolet radiation (UVR)‐induced changes to the dermal ECM are lacking. To investigate the effect of an inflammatory dose of solar‐simulated UVR on human dermal ECM and potential for protection by GTCs in a double‐blind randomized controlled trial. In total, 50 healthy white (Fitzpatrick skin type I–II) adults aged 18–65 years were randomized to a combination of GTCs 540 mg plus vitamin C 50 mg or to placebo twice daily for 12 weeks. The impact of solar‐simulated UVR at 3 × minimal erythema dose on the dermal collagen and elastic fibre networks was assessed by histology and immunohistochemistry in all participants at baseline. The impact of GTC supplementation on UVR‐induced effects was compared between the groups post‐supplementation. The area of papillary dermis covered by collagen and elastic fibres was significantly lower (P < 0.001) in UVR‐exposed skin than in unexposed skin. Significantly lower levels of fibrillin‐rich microfibrils (P = 0.02), fibulin‐2 (P < 0.001) and fibulin‐5 (P < 0.001) were seen in UVR‐exposed than unexposed skin, while procollagen‐1 deposition was significantly higher in UVR‐exposed skin (P = 0.01). Following GTC supplementation, the UVR‐induced change in fibulin‐5 was abrogated in the active group but not the placebo group, with no difference between the two groups for other components. Acute UVR induced significant changes in the human dermal collagen and elastic fibre networks, whereas oral GTCs conferred specific UVR protection to fibulin‐5. Future studies could explore the impact of GTCs on the effects of repeated suberythemal UVR exposure of human skin. Chronic exposure of human skin to ultraviolet radiation (UVR) in sunlight leads to loss and remodelling of dermal extracellular matrix components, acute UVR exposure may also contribute to photodamage. A single sunburn dose of solar‐simulated UVR caused significant reduction in the global collagen and elastic fibre networks, specifically fibrillin‐rich microfibrils, fibulin‐2 and fibulin‐5. Oral supplementation with green tea catechins abrogated the UVR‐induced reduction in fibulin‐5 with no significant effect on other dermal matrix parameters.
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影响因子：
5.6
作者：
Pittayapruek P;Meephansan J;Prapapan O;Komine M;Ohtsuki M
通讯作者：
Ohtsuki M
DOI：
10.1039/c7pp00412e
发表时间：
2018-07-11
期刊：
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
影响因子：
--
作者：
Charoenchon N ;Rhodes LE ;Pilkington SM ;Farrar MD ;Watson REB
通讯作者：
Watson REB
DOI：
10.1016/j.jnutbio.2015.09.001
发表时间：
2016-01
期刊：
The Journal of nutritional biochemistry
影响因子：
--
作者：
Clarke KA;Dew TP;Watson RE;Farrar MD;Osman JE;Nicolaou A;Rhodes LE;Williamson G
通讯作者：
Williamson G
影响因子：
64.8
作者：
Yanagisawa, H;Davis, EC;Olson, EN
通讯作者：
Olson, EN
影响因子：
6.5
作者：
Watson, REB;Griffiths, CEM;Kielty, CM
通讯作者：
Kielty, CM