Expanding the Scope of an Amphoteric Condensed Tannin, Tanfloc, for Antibacterial Coatings.
Expanding the Scope of an Amphoteric Condensed Tannin, Tanfloc, for Antibacterial Coatings.
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DOI：
10.3390/jfb14110554
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发表时间：
2023-11-18
影响因子：
4.8
通讯作者：
中科院分区：
文献类型：
--
作者：
细菌感染是医疗植入物失败的常见模式。本研究旨在开发抗菌聚电解质多层（PEM）涂层，该涂层含有具有固有抗菌活性的植物衍生缩合单宁聚合物（Tanfloc， TAN）。Tanfloc是两性的，在这里我们表明它可以作为聚阴离子或聚阳离子在PEM中使用，从而扩大了它在PEM涂层中使用的可能性。PEMs通常由聚阳离子和聚阴离子形成，其中两种聚合物的官能团（离子）相互络合。然而，使用两性聚合物Tanfloc与弱碱性胺和弱酸性儿茶酚和邻苯三酚基团，使PEM仅使用其一个或另一个官能团形成，使另一个官能团可用来赋予抗菌活性。本研究展示了使用多种反聚电解质（包括三种不同电荷密度的聚阴离子糖胺聚糖）和聚合阳离子N，N，N-三甲基壳聚糖和聚乙烯亚胺)制备的含tan絮的PEMs。利用傅里叶变换表面等离子体共振（FT-SPR）原位监测了PEMs的层层组装，证实了PEMs的层层组装是稳定的。用x射线光电子能谱（XPS）评价表面化学性质，用原子力显微镜（AFM）测定表面粗糙度。在含tanflo的PEMs上培养的细胞LDH释放水平与阴性对照无统计学差异（p > 0.05），证实其无细胞毒性，同时对铜绿假单胞菌（p . aeruginosa）和金黄色葡萄球菌（S. aureus）分别表现出显著的抗粘附和杀菌性能。其抗菌作用主要归因于静电相互作用和Tanfloc的多酚性质。这项工作强调了Tanfloc作为一种抗表面感染的多功能生物材料的潜力。
Bacterial infections are a common mode of failure for medical implants. This study aims to develop antibacterial polyelectrolyte multilayer (PEM) coatings that contain a plant-derived condensed tannin polymer (Tanfloc, TAN) with inherent antimicrobial activity. Tanfloc is amphoteric, and herein we show that it can be used as either a polyanion or a polycation in PEMs, thereby expanding the possibility of its use in PEM coatings. PEMs are ordinarily formed using a polycation and a polyanion, in which the functional (ionic) groups of the two polymers are complexed to each other. However, using the amphoteric polymer Tanfloc with weakly basic amine and weakly acidic catechol and pyrogallol groups enables PEM formation using only one or the other of its functional groups, leaving the other functional group available to impart antibacterial activity. This work demonstrates Tanfloc-containing PEMs using multiple counter-polyelectrolytes including three polyanionic glycosaminoglycans of varying charge density, and the polycations N,N,N-trimethyl chitosan and polyethyleneimine. The layer-by-layer (LbL) assembly of PEMs was monitored using in situ Fourier-transform surface plasmon resonance (FT-SPR), confirming a stable LbL assembly. X-ray photoelectron spectroscopy (XPS) was used to evaluate surface chemistry, and atomic force microscopy (AFM) was used to determine the surface roughness. The LDH release levels from cells cultured on the Tanfloc-containing PEMs were not statistically different from those on the negative control (p > 0.05), confirming their non-cytotoxicity, while exhibiting remarkable antiadhesive and bactericidal properties against Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus), respectively. The antibacterial effects were attributed to electrostatic interactions and Tanfloc’s polyphenolic nature. This work underscores the potential of Tanfloc as a versatile biomaterial for combating infections on surfaces.
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影响因子：
5.4
作者：
Donadio G;Mensitieri F;Santoro V;Parisi V;Bellone ML;De Tommasi N;Izzo V;Dal Piaz F
通讯作者：
Dal Piaz F
DOI：
10.3390/nano12060949
发表时间：
2022-03-14
期刊：
Nanomaterials (Basel, Switzerland)
影响因子：
--
作者：
Díez-Pascual AM;Rahdar A
通讯作者：
Rahdar A
影响因子：
4.9
作者：
Fu, Jinhong;Ji, Jian;Shen, Jiacong
通讯作者：
Shen, Jiacong
DOI：
10.1016/j.ibiod.2018.10.005
发表时间：
2019-01-01
影响因子：
4.8
作者：
Greenhalgh, Richard;Dempsey-Hibbert, Nina C.;Whitehead, Kathryn A.
通讯作者：
Whitehead, Kathryn A.
影响因子：
11.1
作者：
Hameed, Yasir Talib;Idris, Azni;Suja, Fatihah
通讯作者：
Suja, Fatihah