A comparison of the influence of material on in vitro cartilage tissue engineering with PCL, PGS, and POC 3D scaffold architecture seeded with chondrocytes.
A comparison of the influence of material on in vitro cartilage tissue engineering with PCL, PGS, and POC 3D scaffold architecture seeded with chondrocytes.
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DOI：
10.1016/j.biomaterials.2010.01.145
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发表时间：
2010-05
期刊：
影响因子：
14
通讯作者：
Hollister SJ
中科院分区：
文献类型：
--
作者：
Jeong CG;Hollister SJ
本研究的目的是确定具有相同受控结构的支架材料对软骨再生的影响。对相同设计的 3D 聚己内酯 (PCL)、聚癸二酸甘油酯 (PGS) 和聚 (1,8 辛二醇-柠檬酸共聚物) (POC) 支架进行物理表征，并在细胞表型、细胞增殖和分化以及基质生产方面比较组织再生，以找出哪种材料最适合体外软骨再生。 POC 在组织向内生长、基质生成和软骨细胞分化的相对 mRNA 表达 (Col2/Col1) 方面为软骨再生提供了最佳支持。基于其相对较高的去分化 (Col1)、肥大的 mRNA 表达 (Col10) 和较高的基质降解 (MMP13、MMP3) 结果，PGS 被视为对软骨最不利的材料。 PCL 仍然提供了适合细胞活跃的微环境，但它似乎导致支架内软骨细胞去分化 (Col1)，同时许多细胞迁移出，在支架外生长软骨。
The goal of this study was to determine material effects on cartilage regeneration for scaffolds with the same controlled architecture. The 3D polycaprolactone (PCL), poly (glycerol sebacate) (PGS), and poly (1,8 octanediol-co-citrate) (POC) scaffolds of the same design were physically characterized and tissue regeneration in terms of cell phenotype, cellular proliferation and differentiation, and matrix production were compared to find which material would be most optimal for cartilage regeneration in vitro. POC provided the best support for cartilage regeneration in terms of tissue ingrowth, matrix production, and relative mRNA expressions for chondrocyte differentiation (Col2/Col1). PGS was seen as the least favorable material for cartilage based on its relatively high de-differentiation (Col1), hypertrophic mRNA expression (Col10) and high matrix degradation (MMP13, MMP3) results. PCL still provided microenvironments suitable for cells to be active yet it seemed to cause de-differentiation (Col1) of chondrocytes inside the scaffold while many cells migrated out, growing cartilage outside the scaffold.
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影响因子：
14
作者：
Motlagh, Delara;Yang, Jian;Ameer, Guillermo A.
通讯作者：
Ameer, Guillermo A.
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
影响因子：
14
作者：
Li, WJ;Tuli, R;Tuan, RS
通讯作者：
Tuan, RS
影响因子：
4.9
作者：
Kim, Hye-Joung;Lee, Jin-Ho;Im, Gun-Il
通讯作者：
Im, Gun-Il
影响因子：
4.9
作者：
Izquierdo, R.;Garcia-Giralt, N.;Suay, J.
通讯作者：
Suay, J.