Col V siRNA engineered tenocytes for tendon tissue engineering.
Col V siRNA engineered tenocytes for tendon tissue engineering.
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DOI：
10.1371/journal.pone.0021154
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ouyang HW
中科院分区：
文献类型：
--
作者：
Lu P;Zhang GR;Song XH;Zou XH;Wang LL;Ouyang HW
肌腱修复中均匀小的胶原纤维的存在被认为在次优肌腱愈合中起主要作用。胶原V在愈合肌腱中显著升高，并在原纤维形成中起重要作用。本研究的目的是研究胶原V的一个特定的链对Sprague-Dawley大鼠肌腱细胞的原纤维形成的影响，以及Col V siRNA工程肌腱细胞的肌腱组织工程的功效。采用RNA干扰基因治疗和无支架组织工程化肌腱模型。结果表明，无支架组织工程化肌腱具有组织特异性肌腱结构。通过siRNA（Col 5 α1 siRNA、Col 5 α2 siRNA）下调胶原V α1或α2链对I型胶原和核心蛋白聚糖基因表达的影响不同。Col 5 α1 siRNA处理的肌腱细胞胶原纤维变小，形态异常，而Col 5 α2 siRNA处理的肌腱细胞形态与正常肌腱细胞相同。将Col 5 α1 siRNA处理的肌腱细胞与正常肌腱细胞按一定比例共培养，形成的肌腱胶原纤维较大，轮廓相对正常。总之，证明了Col V siRNA工程化的肌腱细胞改善了肌腱组织再生。最佳水平的胶原V在调节胶原纤维形成中至关重要。这可能为未来开发基于细胞和分子生物学的肌腱疾病治疗方法提供基础。
The presence of uniformly small collagen fibrils in tendon repair is believed to play a major role in suboptimal tendon healing. Collagen V is significantly elevated in healing tendons and plays an important role in fibrillogenesis. The objective of this study was to investigate the effect of a particular chain of collagen V on the fibrillogenesis of Sprague-Dawley rat tenocytes, as well as the efficacy of Col V siRNA engineered tenocytes for tendon tissue engineering. RNA interference gene therapy and a scaffold free tissue engineered tendon model were employed. The results showed that scaffold free tissue engineered tendon had tissue-specific tendon structure. Down regulation of collagen V α1 or α2 chains by siRNAs (Col5α1 siRNA, Col5α2 siRNA) had different effects on collagen I and decorin gene expressions. Col5α1 siRNA treated tenocytes had smaller collagen fibrils with abnormal morphology; while those Col5α2 siRNA treated tenocytes had the same morphology as normal tenocytes. Furthermore, it was found that tendons formed by coculture of Col5α1 siRNA treated tenocytes with normal tenocytes at a proper ratio had larger collagen fibrils and relative normal contour. Conclusively, it was demonstrated that Col V siRNA engineered tenocytes improved tendon tissue regeneration. And an optimal level of collagen V is vital in regulating collagen fibrillogenesis. This may provide a basis for future development of novel cellular- and molecular biology-based therapeutics for tendon diseases.
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影响因子：
4.8
作者：
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通讯作者：
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使用针织丝支架结合胶原基质进行韧带再生
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影响因子：
2.4
作者：
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