A C-type lectin collaborates with a CD45 phosphatase homolog to facilitate West Nile virus infection of mosquitoes.
A C-type lectin collaborates with a CD45 phosphatase homolog to facilitate West Nile virus infection of mosquitoes.
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DOI：
10.1016/j.cell.2010.07.038
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发表时间：
2010-09-03
期刊：
影响因子：
64.5
通讯作者：
Fikrig E
中科院分区：
文献类型：
--
作者：
Cheng G;Cox J;Wang P;Krishnan MN;Dai J;Qian F;Anderson JF;Fikrig E
西尼罗河病毒（WNV）是美国最常见的节肢动物传播的黄病毒;然而，参与感染的载体配体尚不清楚。我们现在表明，埃及伊蚊C型凝集素，mosGCTL-1，是由西尼罗河病毒诱导，与西尼罗河病毒在钙依赖性的方式相互作用，并促进感染在体内和体外。在A.埃及伊蚊命名为mosPTP-1，募集mosGCTL-1以使病毒附着于细胞并增强病毒进入。体内实验表明，mosGCTL-1和mosPTP-1作为相同途径的一部分起作用，并且对于蚊子的WNV感染至关重要。在致倦库蚊（Culex quinquefasciatus）中也观察到类似的现象，进一步证明这些基因参与了西尼罗河病毒的感染。在蚊子吸血过程中，WNV感染被mosGCTL-1抗体体内阻断。对黄病毒-节肢动物相互作用的分子理解可能会导致控制病毒在自然界传播的策略。
West Nile virus (WNV) is the most common arthropod-borne flavivirus in the United States; however, the vector ligand(s) that participate in infection are not known. We now show that an Aedes aegypti C-type lectin, mosGCTL-1, is induced by WNV, interacts with WNV in a calcium-dependent manner, and facilitates infection in vivo and in vitro. A mosquito homologue of human CD45 in A. aegypti, designated mosPTP-1, recruits mosGCTL-1 to enable viral attachment to cells, and to enhance viral entry. In vivo experiments show that mosGCTL-1 and mosPTP-1 function as part of the same pathway and are critical for WNV infection of mosquitoes. A similar phenomenon was also observed in Culex quinquefasciatus, a natural vector of WNV, further demonstrating that these genes participate in WNV infection. During the mosquito blood-feeding process, WNV infection was blocked in vivo with mosGCTL-1 antibodies. A molecular understanding of flaviviral-arthropod interactions may lead to strategies to control viral dissemination in nature.
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