The durably resistant rice cultivar Digu activates defence gene expression before the full maturation of Magnaporthe oryzae appressorium.
The durably resistant rice cultivar Digu activates defence gene expression before the full maturation of Magnaporthe oryzae appressorium.
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持久抗性水稻品种 Digu 在 Magnaporthe oryzae appresorium 完全成熟之前激活防御基因表达
DOI：
10.1111/mpp.12286
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发表时间：
2016-04
影响因子：
4.9
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Li W;Liu Y;Wang J;He M;Zhou X;Yang C;Yuan C;Wang J;Chern M;Yin J;Chen W;Ma B;Wang Y;Qin P;Li S;Ronald P;Chen X
由稻瘟病菌引起的稻瘟病是世界上最具破坏性的病害之一。虽然水稻-稻曲病菌互作已被广泛研究，但稻曲病菌入侵期间在附着胞完全成熟之前发生的早期分子事件尚不清楚。在这里，我们报告了持久抗性水稻品种地谷和敏感水稻品种丽江新团黑谷（LTH）对M . novel感染的反应（接种后5，10和20 h，附着胞完全发育之前）的比较转录组学分析。我们发现，这两个水稻品种的转录反应显着不同。基因本体和途径分析表明，许多生物学过程，包括细胞外识别和生物合成的抗氧化剂，萜烯和激素，特异性激活后不久，在Digu。48个编码受体激酶（RKs）的基因在Digu中受到M . cirrhosis感染的显著差异调节。这些基因之一，编码来自LRR VIII-2亚家族的富含亮氨酸重复序列RK的基因，在水稻中过表达时赋予对稻瘟病菌增强的抗性。我们的研究表明，在持久抗性水稻地谷中，在M . pesticides感染后附着胞完全成熟之前发生了许多分子事件，并且膜相关RKs在早期反应中起重要作用。
Rice blast caused by the fungal pathogen M agnaporthe oryzae is one of the most destructive diseases worldwide. Although the rice–M . oryzae interaction has been studied extensively, the early molecular events that occur in rice before full maturation of the appressorium during M . oryzae invasion are unknown. Here, we report a comparative transcriptomics analysis of the durably resistant rice variety Digu and the susceptible rice variety Lijiangxintuanheigu (LTH) in response to infection by M . oryzae (5, 10 and 20 h post‐inoculation, prior to full development of the appressorium). We found that the transcriptional responses differed significantly between these two rice varieties. Gene ontology and pathway analyses revealed that many biological processes, including extracellular recognition and biosynthesis of antioxidants, terpenes and hormones, were specifically activated in Digu shortly after infection. Forty‐eight genes encoding receptor kinases (RKs) were significantly differentially regulated by M . oryzae infection in Digu. One of these genes, LOC _Os08g10300, encoding a leucine‐rich repeat RK from the LRR VIII‐2 subfamily, conferred enhanced resistance to M . oryzae when overexpressed in rice. Our study reveals that a multitude of molecular events occur in the durably resistant rice Digu before the full maturation of the appressorium after M . oryzae infection and that membrane‐associated RKs play important roles in the early response.
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影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
影响因子：
7.2
作者：
He, ZH;He, DZ;Kohorn, BD
通讯作者：
Kohorn, BD
影响因子：
7.4
作者：
Gowda, Malali;Venu, R.-C.;Wang, Guo-Liang
通讯作者：
Wang, Guo-Liang
影响因子：
7.4
作者：
Jantasuriyarat, C;Gowda, M;Wang, GL
通讯作者：
Wang, GL
影响因子：
1.5
作者：
Chen, XW;Li, SG;Zhu, LH
通讯作者：
Zhu, LH