Lassa virus in novel hosts: insights into the epidemiology of lassa virus infections in southern Nigeria.
Lassa virus in novel hosts: insights into the epidemiology of lassa virus infections in southern Nigeria.
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DOI：
10.1080/22221751.2023.2294859
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发表时间：
2024-12
影响因子：
13.2
通讯作者：
中科院分区：
文献类型：
--
作者：
确定造成从动物到人类的溢出事件的各种动物宿主对于理解新出现的传染病的传播模式至关重要，这些传染病构成重大的公共卫生风险。为了更好地表征拉沙病毒（LASV）的潜在动物宿主，我们评估了2021-2022年尼日利亚南部4个报告拉沙热（LF）病例的地点的家畜和非家畜。使用RT-qPCR筛选鸟类、蜥蜴和家养哺乳动物（狗、猪、牛和山羊），并对选定的LASV阳性样品进行全基因组测序以进行谱系鉴定。还通过酶联免疫吸附试验（ELISA）筛选动物是否暴露于LASV。在这些动物中，蜥蜴的PCR阳性率最高。大多数感染动物的样本基因组测序显示LASV亚系2 g。 血清阳性率在牛中最高，在猪中最低。尽管这些额外的宿主在更广泛的病毒宿主背景下可能产生的具体影响仍然未知-特别是与病原体多样性、进化和传播有关-但在生活在确诊的人类LF病例附近的非啮齿类宿主中检测到LASV表明它们在传播过程中作为潜在的宿主参与其中。比较来自人类和动物的病毒基因组以及在环境中传播的病毒基因组的其他流行病学数据对于了解LASV传播动态至关重要，并将最终指导针对这种人畜共患病健康威胁的对策的制定。
Identification of the diverse animal hosts responsible for spill-over events from animals to humans is crucial for comprehending the transmission patterns of emerging infectious diseases, which pose significant public health risks. To better characterize potential animal hosts of Lassa virus (LASV), we assessed domestic and non-domestic animals from 2021–2022 in four locations in southern Nigeria with reported cases of Lassa fever (LF). Birds, lizards, and domestic mammals (dogs, pigs, cattle and goats) were screened using RT-qPCR, and whole genome sequencing was performed for lineage identification on selected LASV positive samples. Animals were also screened for exposure to LASV by enzyme-linked immunosorbent assay (ELISA). Among these animals, lizards had the highest positivity rate by PCR. Genomic sequencing of samples in most infected animals showed sub-lineage 2 g of LASV. Seropositivity was highest among cattle and lowest in pigs. Though the specific impact these additional hosts may have in the broader virus-host context are still unknown – specifically relating to pathogen diversity, evolution, and transmission – the detection of LASV in non-rodent hosts living in proximity to confirmed human LF cases suggests their involvement during transmission as potential reservoirs. Additional epidemiological data comparing viral genomes from humans and animals, as well as those circulating within the environment will be critical in understanding LASV transmission dynamics and will ultimately guide the development of countermeasures for this zoonotic health threat.
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影响因子：
4.8
作者：
Hensley LE;Smith MA;Geisbert JB;Fritz EA;Daddario-DiCaprio KM;Larsen T;Geisbert TW
通讯作者：
Geisbert TW
DOI：
10.4269/ajtmh.16-0675
发表时间：
2017-04
期刊：
The American journal of tropical medicine and hygiene
影响因子：
--
作者：
Bonwitt J;Sáez AM;Lamin J;Ansumana R;Dawson M;Buanie J;Lamin J;Sondufu D;Borchert M;Sahr F;Fichet-Calvet E;Brown H
通讯作者：
Brown H
影响因子：
5.2
作者：
Manning, John T.;Forrester, Naomi;Paessler, Slobodan
通讯作者：
Paessler, Slobodan
影响因子：
16.6
作者：
Klitting, Raphaelle;Kafetzopoulou, Liana E.;Thiery, Wim;Dudas, Gytis;Gryseels, Sophie;Kotamarthi, Anjali;Vrancken, Bram;Gangavarapu, Karthik;Momoh, Mambu;Sandi, John Demby;Goba, Augustine;Alhasan, Foday;Grant, Donald S.;Okogbenin, Sylvanus;Ogbaini-Emovo, Ephraim;Garry, Robert F.;Smither, Allison R.;Zeller, Mark;Pauthner, Matthias G.;McGraw, Michelle;Hughes, Laura D.;Duraffour, Sophie;Guenther, Stephan;Suchard, Marc A.;Lemey, Philippe;Andersen, Kristian G.;Dellicour, Simon
通讯作者：
Dellicour, Simon
影响因子：
2.6
作者：
Bjustrom-Kraft J;Woodard K;Giménez-Lirola L;Rotolo M;Wang C;Sun Y;Lasley P;Zhang J;Baum D;Gauger P;Main R;Zimmerman J
通讯作者：
Zimmerman J