Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation.
Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation.
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基于成像椭圆光度法和 AFM 显微确认的生物传感器检测噬菌体 M13KO7。
DOI：
10.1016/j.virusres.2008.11.010
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发表时间：
2009-03
期刊：
影响因子：
5
通讯作者：
Jin G
中科院分区：
文献类型：
--
作者：
Qi C;Lin Y;Feng J;Wang ZH;Zhu CF;Meng YH;Yan XY;Wan LJ;Jin G
快速检测和识别病原体对于最大限度地减少疾病的转移和传播非常重要。基于椭圆偏振成像技术（BIE），建立了一种检测噬菌体M13 KO 7的免标记复合生物传感器。用醛对硅片表面进行修饰，利用微流控系统可以将蛋白质以阵列形式均匀地同时图案化在硅片表面。通过亲和素与生物素的相互作用，将亲和素固定在噬菌体M13 KO 7表面，用于生物素-抗M13抗体的固定，作为抗噬菌体M13 KO 7的配体。当噬菌体M13 KO 7溶液通过表面时，噬菌体M13 KO 7被配体特异性捕获，导致抗M13结合噬菌体M13 KO 7层的质量表面浓度显著增加，这可以通过成像椭圆偏振法检测，灵敏度为109 pfu/ml。此外，原子力显微镜也被用来确认噬菌体M13 KO 7已被表面上的配体直接捕获的事实。结果表明，BIE可直接检测噬菌体M13 KO 7，在病毒检测领域具有应用潜力。
A rapid detection and identification of pathogens is important for minimizing transfer and spread of disease. A label-free and multiplex biosensor based on imaging ellipsometry (BIE) had been developed for the detection of phage M13KO7. The surface of silicon wafer is modified with aldehyde, and proteins can be patterned homogeneously and simultaneously on the surface of silicon wafer in an array format by a microfluidic system. Avidin is immobilized on the surface for biotin-anti-M13 immobilization by means of interaction between avidin and biotin, which will serve as ligand against phage M13KO7. Phages M13KO7 are specifically captured by the ligand when phage M13KO7 solution passes over the surface, resulting in a significant increase of mass surface concentration of the anti-M13 binding phage M13KO7 layer, which could be detected by imaging ellipsometry with a sensitivity of 109 pfu/ml. Moreover, atomic force microscopy is also used to confirm the fact that phage M13KO7 has been directly captured by ligands on the surface. It indicates that BIE is competent for direct detection of phage M13KO7 and has potential in the field of virus detection.
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DOI：
10.1016/s1872-2075(06)60060-5
发表时间：
2006-09
期刊：
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
影响因子：
--
作者：
Qi C;Feng J;Wang ZH;Meng YH;Yan XY;Jin G
通讯作者：
Jin G
影响因子：
2.9
作者：
JIN, G;TENGVALL, P;ARWIN, H
通讯作者：
ARWIN, H
影响因子：
12.6
作者：
Bisoffi, M.;Hjelle, B.;Larson, R. S.
通讯作者：
Larson, R. S.
DOI：
10.1016/j.bbrc.2003.10.022
发表时间：
2003-11-14
影响因子：
3.1
作者：
Nettikadan, SR;Johnson, JC;Henderson, E
通讯作者：
Henderson, E
影响因子：
12.6
作者：
Uttenthaler, E;Schräml, M;Drost, S
通讯作者：
Drost, S