Heat Shock Transcription Factor CgHSF1 Is Required for Melanin Biosynthesis, Appressorium Formation, and Pathogenicity in Colletotrichum gloeosporioides.
Heat Shock Transcription Factor CgHSF1 Is Required for Melanin Biosynthesis, Appressorium Formation, and Pathogenicity in Colletotrichum gloeosporioides.
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DOI：
10.3390/jof8020175
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发表时间：
2022-02-11
期刊：
影响因子：
--
通讯作者：
An B
中科院分区：
文献类型：
--
作者：
Gao X;Wang Q;Feng Q;Zhang B;He C;Luo H;An B
热休克转录因子（HSFs）是转录调节因子家族中的一员。虽然HSFs在控制分子伴侣热激蛋白的转录和抗胁迫方面的功能已经得到了很好的证实，但它们对植物病原真菌致病性的影响仍然未知。本研究分析了CgHSF1在炭疽菌致病性中的作用，并探讨了其致病机制。未能产生Cghsf1敲除突变体表明该基因对真菌的生存能力至关重要。然后，通过在其编码序列之前插入亚硝酸盐还原酶基因（PniiA）的抑制性启动子，实现了Cghsf1的遗传缺失。该突变体的致病性显著降低，附着胞形成受到抑制，黑色素生物合成严重缺陷。此外，四个黑色素合成基因被确定为CgHSF1的直接靶点。总之，这项工作突出了CgHSF1通过黑色素生物合成的转录激活在真菌致病性中的作用。我们的研究扩展了对真菌HSF 1蛋白的理解，特别是它们参与致病性。
Heat shock transcription factors (HSFs) are a family of transcription regulators. Although HSFs’ functions in controlling the transcription of the molecular chaperone heat shock proteins and resistance to stresses are well established, their effects on the pathogenicity of plant pathogenic fungi remain unknown. In this study, we analyze the role of CgHSF1 in the pathogenicity of Colletotrichum gloeosporioides and investigate the underlying mechanism. Failure to generate the Cghsf1 knock-out mutant suggested that the gene is essential for the viability of the fungus. Then, genetic depletion of the Cghsf1 was achieved by inserting the repressive promoter of nitrite reductase gene (PniiA) before its coding sequence. The mutant showed significantly decrease in the pathogenicity repression of appressorium formation, and severe defects in melanin biosynthesis. Moreover, four melanin synthetic genes were identified as direct targets of CgHSF1. Taken together, this work highlights the role of CgHSF1 in fungal pathogenicity via the transcriptional activation of melanin biosynthesis. Our study extends the understanding of fungal HSF1 proteins, especially their involvement in pathogenicity.
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影响因子：
6.7
作者：
Michielse CB;van Wijk R;Reijnen L;Manders EM;Boas S;Olivain C;Alabouvette C;Rep M
通讯作者：
Rep M
影响因子：
7.4
作者：
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通讯作者：
LOW, PS
DOI：
10.1196/annals.1391.005
发表时间：
2007-01-01
期刊：
STRESS RESPONSES IN BIOLOGY AND MEDICINE
影响因子：
--
作者：
Akerfelt, Malin;Trouillet, Diane;Sistonen, Lea
通讯作者：
Sistonen, Lea
影响因子：
3.6
作者：
Nicholls S;Leach MD;Priest CL;Brown AJ
通讯作者：
Brown AJ
DOI：
10.1038/nrm.2017.73
发表时间：
2018-01
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Gomez-Pastor R;Burchfiel ET;Thiele DJ
通讯作者：
Thiele DJ