SFRP2/DPP4 and FMO1/LSP1 Define Major Fibroblast Populations in Human Skin.
SFRP2/DPP4 and FMO1/LSP1 Define Major Fibroblast Populations in Human Skin.
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DOI：
10.1016/j.jid.2017.09.045
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Lafyatis R
中科院分区：
文献类型：
--
作者：
Tabib T;Morse C;Wang T;Chen W;Lafyatis R
成纤维细胞产生基质，调节炎症，介导修复过程，并作为多能间充质细胞。通过单细胞RNA-seq（scRNA-seq）分析消化的正常人皮肤，我们探索了不同的成纤维细胞群体。来自六个活检组织的scRNA-seq数据的T分布随机邻居嵌入和聚类揭示了两个主要的成纤维细胞群体，由不同的基因定义，包括SFRP 2和FMO 1，仅由这两个主要的成纤维细胞群体表达。在SFRP 2和FMO 1群体中的每一个以及五个次要成纤维细胞群体中定义了进一步的亚群，每个群体表达离散基因：CRABP 1、COL 11 A1、FMO 2、PRG 4或C2 ORF 40。免疫荧光染色证实，SFRP 2和FMO 1定义的细胞类型的显着不同的形态。SFRP 2+成纤维细胞小，细长，分布在胶原束之间。FMO 1+成纤维细胞较大，分布在间质和血管周围位置。SFRP 2+、FMO 1+和COL 11 A1+成纤维细胞的差异基因表达分别表明在基质沉积、炎症细胞保留和结缔组织细胞分化中的作用。
Fibroblasts produce matrix, regulate inflammation, mediate reparative processes, and serve as pluripotent mesenchymal cells. Analyzing digested normal human skin by single cell RNA-seq (scRNA-seq), we explored different fibroblast populations. T-distributed stochastic neighbor embedding and clustering of scRNA-seq data from six biopsies revealed two major fibroblast populations, defined by distinct genes, including SFRP2 and FMO1, expressed exclusively by these two major fibroblast populations. Further subpopulations were defined within each of the SFRP2 and FMO1 populations, as well as five minor fibroblast populations, each expressing discrete genes: CRABP1, COL11A1, FMO2, PRG4 or C2ORF40. Immunofluorescent staining confirmed that SFRP2 and FMO1 define cell types of dramatically different morphology. SFRP2+ fibroblasts were small, elongated, and distributed between collagen bundles. FMO1+ fibroblasts were larger, and distributed in both interstitial and perivascular locations. Differential gene expression by SFRP2+, FMO1+ and COL11A1+ fibroblasts suggests roles in matrix deposition, inflammatory cell retention, and connective tissue cell differentiation, respectively.
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影响因子：
6
作者：
Dykstra JA;Facile T;Patrick RJ;Francis KR;Milanovich S;Weimer JM;Kota DJ
通讯作者：
Kota DJ
影响因子：
64.5
作者：
Fujiwara H;Ferreira M;Donati G;Marciano DK;Linton JM;Sato Y;Hartner A;Sekiguchi K;Reichardt LF;Watt FM
通讯作者：
Watt FM
DOI：
10.1126/science.aaa2151
发表时间：
2015-04-17
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rinkevich Y;Walmsley GG;Hu MS;Maan ZN;Newman AM;Drukker M;Januszyk M;Krampitz GW;Gurtner GC;Lorenz HP;Weissman IL;Longaker MT
通讯作者：
Longaker MT
影响因子：
7.3
作者：
Barone F;Gardner DH;Nayar S;Steinthal N;Buckley CD;Luther SA
通讯作者：
Luther SA
影响因子：
64.5
作者：
Rodeheffer, Matthew S.;Birsoy, Kivanc;Friedman, Jeffrey M.
通讯作者：
Friedman, Jeffrey M.