On-chip integrated graphene aptasensor with portable readout for fast and label-free COVID-19 detection in virus transport medium.
On-chip integrated graphene aptasensor with portable readout for fast and label-free COVID-19 detection in virus transport medium.
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DOI：
10.1039/d2sd00076h
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发表时间：
2022-07-14
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石墨烯场效应晶体管（GFET）生物传感器由于大的表面体积比和费米能级对表面附近带电生物分子的存在的高灵敏度而表现出高灵敏度。对于大多数报道的GFET生物传感器，使用笨重的外部参比电极，这妨碍了它们的全尺寸芯片集成，并且导致每次测试的成本更高。在这项研究中，具有芯片上集成液体电极的GFET阵列被用于COVID-19检测，并被抗体或适体功能化以选择性地结合SARS-CoV-2的刺突蛋白。在适体功能化的GFET（适体传感器，Apt-GFET）的情况下，对于临床转运介质中的病毒样颗粒（VLP），实现的检测限（LOD）为约IO 3个颗粒/mL，优于Ab-GFET生物传感器对应物。此外，适体传感器实现了血清中COVID-19中和抗体的LOD为160 aM。传感器被发现具有高度选择性，快速（样品到结果在几分钟内）和稳定（低设备间信号变化;相对标准偏差低于0.5%）。一个自制的便携式读出电子单元被用于同时实时测量每个芯片的12个GFET。我们成功演示了便携式GFET生物传感平台，在传染病检测和其他医疗保健应用方面具有很高的潜力。芯片上集成的石墨烯场效应晶体管（GFET）为基础的适体传感器开发与便携式读出灵敏和特异性的病毒检测。
Graphene field-effect transistor (GFET) biosensors exhibit high sensitivity due to a large surface-to-volume ratio and the high sensitivity of the Fermi level to the presence of charged biomolecules near the surface. For most reported GFET biosensors, bulky external reference electrodes are used which prevent their full-scale chip integration and contribute to higher costs per test. In this study, GFET arrays with on-chip integrated liquid electrodes were employed for COVID-19 detection and functionalized with either antibody or aptamer to selectively bind the spike proteins of SARS-CoV-2. In the case of the aptamer-functionalized GFET (aptasensor, Apt-GFET), the limit-of-detection (LOD) achieved was about 103 particles per mL for virus-like particles (VLPs) in clinical transport medium, outperforming the Ab-GFET biosensor counterpart. In addition, the aptasensor achieved a LOD of 160 aM for COVID-19 neutralizing antibodies in serum. The sensors were found to be highly selective, fast (sample-to-result within minutes), and stable (low device-to-device signal variation; relative standard deviations below 0.5%). A home-built portable readout electronic unit was employed for simultaneous real-time measurements of 12 GFETs per chip. Our successful demonstration of a portable GFET biosensing platform has high potential for infectious disease detection and other health-care applications. On-chip integrated graphene field-effect transistor (GFET)-based aptasensor was developed with portable readouts for sensitive and specific virus detection.
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