Molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus among children with respiratory tract infections in southwest China
Molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus among children with respiratory tract infections in southwest China
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西南地区呼吸道感染儿童金黄色葡萄球菌分子特征及药敏分析
DOI：
10.1007/s12519-019-00317-4
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发表时间：
2019-10
影响因子：
8.7
通讯作者：
Su‑Yun Qian
中科院分区：
文献类型：
--
作者：
Chen Sun;Qing Wang;Wen‑Ting Li;De‑Nian Wen;Chang‑Hui Chen;Xin Yang;Wei Shi;Qing‑Hong Meng;Kai‑Hu Yao;Su‑Yun Qian
背景：普通儿科病房和县级医院金黄色葡萄球菌的分子特征及药敏结果在国内报道较少。方法：从2015年中江、酉阳两县呼吸道感染住院患儿中分离出金黄色葡萄球菌。根据多位点序列、葡萄球菌蛋白A、辅助基因调节子(Agr)和葡萄球菌盒式染色体mec[SCCmec，仅对耐甲氧西林金黄色葡萄球菌(MRSA)]进行分型。聚合酶链式反应筛选21个超抗原(SAG)基因和潘通-瓦伦丁杀白素(PVL)基因。药敏试验采用E试验。结果：共纳入2136名儿童。总体来看，125例(5.9%)儿童携带金黄色葡萄球菌，其中MRSA占42.4%。耐甲氧西林金黄色葡萄球菌(MSSA)以ST59-SCCmec IV型-t437-agr组为主(58.5%)，以ST188-t189-agr组为主(22.2%)。MRSA和MSSA的PVL携带率分别为15.1%和9.7%(P=0.4112)。约96.8%的金黄色葡萄球菌至少有一个SAG基因阳性。在ST59显性克隆中，最常见的SAG基因图谱为seb-sek-seq(42.8%)。所有分离的金黄色葡萄球菌均对青霉素和红霉素耐药(最低抑菌浓度90%对青霉素和红霉素分别为32和256 mg/L)，但对其他非β-内酰胺类抗生素敏感。结论：中国所在县级医院呼吸道感染患儿中金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌的检出率较高。ST59-SCCmec IV型-T437-agr组为MRSA的优势克隆。金黄色葡萄球菌对青霉素和红霉素具有较高的耐药性。
Background:The molecular characteristics and antimicrobial susceptibility of Staphylococcus aureus (S. aureus) in general pediatric wards and county-level hospitals were rarely reported in China.Methods:Staphylococcus aureus was isolated from children hospitalized with respiratory tract infection (RTI) in Zhongjiang and Youyang counties in 2015. All isolates were typed by multilocus sequence, staphylococcal protein A, accessory gene regulator (agr), and staphylococcal cassette chromosome mec [SCCmec, for methicillin-resistant S. aureus (MRSA) only]. Polymerase chain reaction was used to screen 21 super-antigen (SAg) genes and panton-valentine leukocidin (pvl). Antimicrobial susceptibility testing was performed by E test.Results:A total of 2136 children were enrolled. Overall, 125 (5.9%) children carried S. aureus, among which MRSA accounted for 42.4%. ST59-SCCmec type IV-t437-agr group I (58.5%) was the most prevalent genotype in MRSA, and ST188-t189-agr group I (22.2%) was the top genotype in methicillin-sensitive S. aureus (MSSA). The pvl carriage rate in MRSA and MSSA was 15.1% and 9.7%, respectively (P = 0.4112). About 96.8% of S. aureus isolates were positive for at least one SAg gene. The most common SAg gene profile in the dominant ST59 clone was seb-sek-seq (42.8%). All S. aureus isolates were resistant to penicillin and erythromycin (minimum inhibitory concentration 90 was > 32 and 256 mg/L to penicillin and erythromycin, respectively), but usually susceptible to other tested non-β-lactam antimicrobials.Conclusions:Staphylococcus aureus and MRSA were detected with a high frequency in children with RTI in county-level hospitals of China. ST59-SCCmec type IV-t437-agr group I was the dominant MRSA clone. The S. aureus isolates exhibited high resistance to penicillin and erythromycin.
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影响因子：
2.2
作者：
Yang Qin;Feiqiu Wen;Yuejie Zheng;R. Zhao;Q. Hu;Renli Zhang
通讯作者：
Yang Qin;Feiqiu Wen;Yuejie Zheng;R. Zhao;Q. Hu;Renli Zhang
影响因子：
6.1
作者：
Zollner;Wichelhaus;Hartung;Von Mallinckrodt;Wagner;Brade;Kaufmann
通讯作者：
Zollner;Wichelhaus;Hartung;Von Mallinckrodt;Wagner;Brade;Kaufmann
影响因子：
2
作者：
Hesje CK;Sanfilippo CM;Haas W;Morris TW
通讯作者：
Morris TW
影响因子：
4.6
作者：
Yunjia Song;Lanqing Cui;Y. Lv;Yun Li;F. Xue
通讯作者：
Yunjia Song;Lanqing Cui;Y. Lv;Yun Li;F. Xue
影响因子：
3.7
作者：
Laux, Claudia;Peschel, Andreas;Krismer, Bernhard
通讯作者：
Krismer, Bernhard