Seed-Specific Overexpression of SPL12 and IPA1 Improves Seed Dormancy and Grain Size in Rice.

Seed-Specific Overexpression of SPL12 and IPA1 Improves Seed Dormancy and Grain Size in Rice.
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SPL12 和 IPA1 的种子特异性过表达可改善水稻种子休眠和籽粒大小

DOI:
10.3389/fpls.2020.532771
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发表时间:
2020
影响因子:
5.6
通讯作者:
Liu S
Liu S
中科院分区:
生物学2区
文献类型:
--
作者:
Qin M;Zhang Y;Yang Y;Miao C;Liu S

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收获前发芽(PHS)往往导致谷物产量和品质下降,是谷物生产中的一个主要问题。改善种子休眠会抑制PHS。在这里,我们表明,种子特异性过表达的两个SQUAMOSA-PROMOTER结合蛋白样(SPL)基因SPL 12和IPA 1增强种子休眠和抑制PHS没有显着的影响,在水稻芽结构。此外,种子特异性过表达IPA 1还增加了晶粒尺寸,从而提高了晶粒生产力。此外,我们的研究结果表明,SPL 12通过直接调控赤霉素(GA)途径中的许多基因增强种子休眠。本研究为抑制小穗病提供了一种有效的方法,并将有助于培育优良的作物品种。
Pre-harvest sprouting (PHS) often results in reduced grain yield and quality and is a major problem in cereal production. Improved seed dormancy would inhibit PHS. Here we show that seed-specific overexpression of two SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) genes SPL12 and IPA1 enhances seed dormancy and inhibits PHS without noticeable effects on shoot architecture in rice. In addition, seed-specific overexpression of IPA1 also increases grain size and thus improves grain productivity. Furthermore, our results suggest that SPL12 enhances the seed dormancy through directly regulating many genes in the gibberellin (GA) pathway. This research provides an efficient method to suppress PHS and will facilitate breeding elite crop varieties.
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