Pervasive interactions of Sa and Sb loci cause high pollen sterility and abrupt changes in gene expression during meiosis that could be overcome by double neutral genes in autotetraploid rice.
Pervasive interactions of Sa and Sb loci cause high pollen sterility and abrupt changes in gene expression during meiosis that could be overcome by double neutral genes in autotetraploid rice.
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Sa 和 Sb 位点的普遍相互作用导致高花粉不育性和减数分裂期间基因表达的突然变化，这可以通过同源四倍体水稻中的双中性基因来克服
DOI：
10.1186/s12284-017-0188-8
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发表时间：
2017-12-02
期刊：
影响因子：
--
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Wu J;Chen L;Shahid MQ;Chen M;Dong Q;Li J;Xu X;Liu X
背景：水稻亚种间同源四倍体杂种具有较高的杂交活力；然而，花粉的低育性是阻碍其商业化利用的关键因素。我们的前期研究表明，多倍体可以增加水稻的多位点相互作用，导致高花粉败育。然而，对花粉不育位点在亚种间杂交中的关键作用知之甚少。以台中65- 4x近等基因系为材料，在不同花粉不育位点培育出杂合型自四倍体水稻杂交种SiSiSjSj。此外，利用携带双中性基因的同源四倍体系sann和Sbn，评估其对育性恢复的影响。结果：细胞学研究表明，同源四倍体水稻花粉不育位点Sa和Sb的有害遗传互作导致减数分裂中期花粉不育性增高（76.83%）和染色体行为异常（24.59%）。转录组分析显示，在Sa和Sb花粉不育位点普遍互作的杂交种中有1092个差异表达基因（DEG），其中大部分基因（约83%）表达下调。其中60个基因与转录调控相关，18个基因被标注为减数分裂相关基因。对携带双中性基因的同源四倍体水稻杂交组合sann和Sbn的花粉育性分析显示花粉育性正常，转录组分析显示不同杂交组合的DEG数量差异不显著。结论：本研究揭示了Sa和Sb花粉不育位点的普遍相互作用导致同源四倍体杂交种的高不育性，导致减数分裂相关基因和转录调节因子的下调。此外，我们还发现同源四倍体水稻杂交种可以通过双中性基因sann和Sbn来克服不育性。本研究为水稻杂种优势的利用提供了有力的证据。
Background:Intersubspecific autotetraploid rice hybrids possess high hybrid vigor; however, low pollen fertility is a critical hindrance in its commercial utilization. Our previous study demonstrated that polyploidy could increase the multi-loci interaction and cause high pollen abortion in autotetraploid rice hybrids. However, there is little known about the critical role of pollen sterility locus or loci in the intersubspecific hybrids. We developed autotetraploid rice hybrids harboring heterozygous genotypes (SiSiSjSj) at different pollen sterility loci by using the near isogenic lines of Taichung65-4×. Moreover, autotetraploid lines carrying double neutral genes, Sanand Sbn, were used to assess their effect on fertility restoration.Results:Cytological studies showed that the deleterious genetic interactions at Sa and Sb pollen sterility loci resulted in higher pollen sterility (76.83%) and abnormal chromosome behavior (24.59%) at metaphase I of meiosis in autotetraploid rice hybrids. Transcriptome analysis revealed 1092 differentially expressed genes (DEG) in a hybrid with the pervasive interactions at Sa and Sb pollen sterility loci, and most of the genes (about 83%) exhibited down regulation. Of the DEG, 60 were associated with transcription regulation and 18 genes were annotated as meiosis-related genes. Analysis on the hybrids developed by using autotetraploid rice harboring double neutral genes, Sanand Sbn, revealed normal pollen fertility, and transcriptome analysis showed non-significant difference in number of DEG among different hybrids.Conclusions:Our finding revealed that pervasive interactions at Sa and Sb pollen sterility loci cause high sterility in the autotetraploid hybrids that lead to the down-regulation of important meiosis-related genes and transcription regulation factors. Moreover, we also found that the hybrids sterility could be overcome by double neutral genes, Sanand Sbn, in autotetraploid rice hybrids. The present study provided a strong evidence for the utilization of heterosis in autotetraploid rice hybrids.
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