Oxytocin Receptors in the Mouse Centrally-projecting Edinger-Westphal Nucleus and their Potential Functional Significance for Thermoregulation.
Oxytocin Receptors in the Mouse Centrally-projecting Edinger-Westphal Nucleus and their Potential Functional Significance for Thermoregulation.
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DOI：
10.1016/j.neuroscience.2022.07.002
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发表时间：
2022-08-21
期刊：
影响因子：
3.3
通讯作者：
Ryabinin, Andrey E.
中科院分区：
文献类型：
--
作者：
Li, Ju;Ryabinin, Andrey E.
中央投射的Edinger-Westphal核（EWcp）已被证明有助于调节多种功能，包括对压力和恐惧，注意力，食物消耗，成瘾，体温和母性行为的反应。然而，通过EWcp参与调节这些行为的受体仍然缺乏特征。另一方面，催产素肽（OXT）也已知调节大量的生理反应和行为。在这里，我们表明，mRNA编码OXT受体（OXTR）表达在EWcp的男性和女性C57 BL/6 J小鼠。这些受体存在于含尿皮质素1（Ucn）mRNA的神经元上，并且在较小程度上存在于EWcp中表达EWcp的囊泡谷氨酸转运体2（VEGF 2）mRNA的神经元上。使用RNAscope原位杂交，我们发现，神经元含有UCN和VEGF 2 mRNA是两个混合的，但独立的亚群在EWCP和表征其与其他群体的神经元在这个核附近的关系。使用免疫组织化学，我们表明，腹腔内（IP）管理的OXT可以诱导FOS的Oxtr含有神经元，这表明这些受体的EWcp神经元的功能。一项随访研究显示，注射OXT（2.3或7.7 mg/kg，IP）伴随体温降低。由于已知EWcp参与体温调节，我们假设OXT对体温的影响可能通过EWcp介导。EWcp中的OXTR对EWcp和OXT的各种功能的调节的贡献需要被破译。
The centrally-projecting Edinger-Westphal nucleus (EWcp) has been shown to contribute to regulation of multiple functions, including responses to stress and fear, attention, food consumption, addiction, body temperature and maternal behaviors. However, receptors involved in regulation of these behaviors through EWcp remain poorly characterized. On the other hand, the oxytocin peptide (OXT) is also known to regulate a substantial number of physiological responses and behaviors. Here we show that mRNA encoding OXT receptors (Oxtr) is expressed in EWcp of male and female C57BL/6J mice. These receptors are present on urocortin 1 (Ucn) mRNA-containing neurons and, to a lesser extent, on neurons in EWcp expressing the vesicular glutamate transporter 2 (Vglut2) mRNA of EWcp. Using RNAscope in situ hybridization, we show that neurons containing Ucn and Vglut2 mRNAs are two intermingled, but independent subpopulations in EWcp and characterize their relationship with other populations of neurons in the vicinity of this nucleus. Using immunohistochemistry, we show that intraperitoneal (IP) administration of OXT can induce FOS in Oxtr-containing neurons, suggesting that these receptors on EWcp neurons are functional. A follow up study showed that injection of OXT (2.3 or 7.7 mg/kg, IP) is accompanied by a decrease in body temperature. Since EWcp is known to be involved in regulation of body temperature, we hypothesize that OXT’s effects on body temperature could be mediated through the EWcp. The contribution of OXTR in EWcp to regulation of various functions of EWcp and OXT needs to be deciphered.
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影响因子：
2.9
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