The Dorsocross T-box transcription factors promote tissue morphogenesis in the Drosophila wing imaginal disc
The Dorsocross T-box transcription factors promote tissue morphogenesis in the Drosophila wing imaginal disc
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Dorsocross T-box转录因子促进果蝇翼成虫盘中的组织形态发生
DOI：
10.1242/dev.079384
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发表时间：
2012-08
期刊：
影响因子：
4.6
通讯作者：
Shen, Jie
中科院分区：
文献类型：
--
作者：
Sui, Liyuan;Pflugfelder, Gert O.;Shen, Jie
在幼虫第三龄期间，果蝇翼成虫盘通过形成几个深的顶端褶皱而细分为诺托姆、铰链和叶片区域。这些细分的分子机制以及变态过程中随后形态发生过程的启动人们知之甚少。在这里，我们证明 Dorsocross (Doc) T-box 基因促进上皮折叠的进展，不仅将翼盘的铰链和叶片区域分开，而且还通过改变细胞形状和弯曲翼盘来促进变质发育。我们发现 Doc 表达受到两种抑制剂（Vestigial 和 Homothorax）的限制，导致形成两条狭窄的 Doc 条纹，其中形成了分隔铰链和叶片的折叠。 Doc突变克隆阻止了幼虫阶段这些褶皱的横向延伸和加深，并延迟了蛹阶段早期的翼盘弯曲。异位 Doc 表达足以通过引起顶端微管网的基底外侧重新分布和细胞缩短来产生深的顶端褶皱。内源性叶片/铰链折叠和异位 Doc 表达引起的折叠的细胞均表达基质金属蛋白酶 2 (Mmp2)。在这些折叠中，整合素和细胞外基质蛋白被耗尽。沿着叶片/铰链折叠过度表达 Doc 会导致翅盘早熟弯曲，这可以通过共表达 MMP2RNAi 来抑制。
The Drosophila wing imaginal disc is subdivided into notum, hinge and blade territories during the third larval instar by formation of several deep apical folds. The molecular mechanisms of these subdivisions and the subsequent initiation of morphogenic processes during metamorphosis are poorly understood. Here, we demonstrate that the Dorsocross (Doc) T-box genes promote the progression of epithelial folds that not only separate the hinge and blade regions of the wing disc but also contribute to metamorphic development by changing cell shapes and bending the wing disc. We found that Doc expression was restricted by two inhibitors, Vestigial and Homothorax, leading to two narrow Doc stripes where the folds separating hinge and blade are forming. Doc mutant clones prevented the lateral extension and deepening of these folds at the larval stage and delayed wing disc bending in the early pupal stage. Ectopic Doc expression was sufficient to generate deep apical folds by causing a basolateral redistribution of the apical microtubule web and a shortening of cells. Cells of both the endogenous blade/hinge folds and of folds elicited by ectopic Doc expression expressed Matrix metalloproteinase 2 (Mmp2). In these folds, integrins and extracellular matrix proteins were depleted. Overexpression of Doc along the blade/hinge folds caused precocious wing disc bending, which could be suppressed by co-expressing MMP2RNAi.
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DOI：
10.1517/13543776.3.12.1797
发表时间：
2000-04
期刊：
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
影响因子：
--
作者：
M. Nakajima
通讯作者：
M. Nakajima
影响因子：
56.9
作者：
Shen, J;Dahmann, C
通讯作者：
Dahmann, C
影响因子：
6.6
作者：
Godenschwege, TA;Pohar, N;Buchner, E
通讯作者：
Buchner, E
影响因子：
9.2
作者：
Klein, T;Couso, JP;Arias, AM
通讯作者：
Arias, AM
影响因子：
2.6
作者：
K. Soanes;Julie O. MacKay;N. Coré;T. Heslip;S. Kerridge;J. Bell
通讯作者：
K. Soanes;Julie O. MacKay;N. Coré;T. Heslip;S. Kerridge;J. Bell