Metabolomics Analysis of Different Tissues of Lonicera japonica Thunb. Based on Liquid Chromatography with Mass Spectrometry.
Metabolomics Analysis of Different Tissues of Lonicera japonica Thunb. Based on Liquid Chromatography with Mass Spectrometry.
复制标题
金银花不同组织的代谢组学分析。
DOI：
10.3390/metabo13020186
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发表时间：
2023-01-27
期刊：
影响因子：
4.1
通讯作者：
中科院分区：
文献类型：
--
作者：
黄藤(LJT)在亚洲国家被广泛用作药物或食品添加剂已有数千年的历史。花蕾通常可药用，而其他组织被忽略。然而，据报道，花、叶和茎也具有抗菌、抗炎和抗氧化作用。本研究采用非靶向代谢组学方法，以液相色谱-质谱联用技术为基础，研究了枸杞不同组织（花、花蕾、茎、叶）间的代谢差异。共鉴定出171种代谢产物，其中黄酮类28种，酚酸类35种，环烯醚萜类43种，氨基酸类9种，核苷酸类6种，脂肪酸类16种，脂类22种，其它12种。发现了4个新的次生代谢产物。叶和茎中未检测到部分黄酮类化合物和环烯醚萜类化合物。主成分分析表明，四种不同的组织之间的差异显着。在花/花蕾、叶/花蕾、茎/花蕾之间分别发现了27、81、113个差异代谢产物。初级代谢产物在花和花蕾中含量较高。黄酮类化合物主要积累在叶片中，黄酮醇类化合物主要积累在花蕾中，酰化黄酮醇苷类化合物主要积累在花中。大多数酚酸类物质在花或花蕾中含量较高，而酚酸糖苷类物质在花蕾中含量显著较高。环烯醚萜类化合物在花和花蕾中的含量也较高。这些结果为深入了解枸杞子在不同组织中的代谢变化提供了新的思路，为枸杞子的综合利用奠定了理论基础。
Lonicera japonica Thunb. (LJT) has been widely used as medicines or food additives in Asian countries for thousands of years. The flower buds are often medicinally used, and the other tissues are ignored. However, flowers, leaves and stems have also been reported to have antimicrobial, anti-inflammatory and antioxidant effects. In the current study, un-targeted metabolomics analysis was performed to investigate the metabolic difference among different tissues (flowers, flower buds, stems and leaves) of LJT based on liquid chromatography with mass spectrometry. A total of 171 metabolites were identified, including 28 flavonoids, 35 phenolic acids, 43 iridoids, 9 amino acids, 6 nucleotides, 16 fatty acids, 22 lipids and 12 others. Four new secondary metabolites were discovered. Some flavonoids and iridoids were not detected in leaves and stems. Principal component analysis showed significant differences among four different tissues. Some 27, 81, 113 differential metabolites were found between flowers/flower buds, leaves/flower buds, stems/flower buds, respectively. Primary metabolites showed a higher content in the flowers and flower buds. For the flavonoids, flavones were mainly accumulated in the leaves, flavonols were mainly accumulated in the flower buds, and acylated flavonol glucosides were mainly accumulated in the flowers. Most phenolic acids showed a higher content in the flowers or flower buds, while phenolic acid-glucosides showed significantly higher content in the flower buds. The most abundant iridoids in the LJT also showed a higher content in the flowers and flower buds. These results can provide new insights into the understanding of the metabolites changes in different tissues, and lay a theoretical foundation for the comprehensive utilization of LJT.
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影响因子：
3.9
作者：
Fan R;Peng C;Zhang X;Qiu D;Mao G;Lu Y;Zeng J
通讯作者：
Zeng J
影响因子：
3.8
作者：
Kou, Yaoyao;Li, Zhihao;Yang, Tong;Shen, Xue;Wang, Xin;Li, Haopeng;Zhou, Kun;Li, Luyao;Xia, Zhaodi;Zheng, Xiaohui;Zhao, Ye
通讯作者：
Zhao, Ye
DOI：
10.1016/j.jpba.2022.114906
发表时间：
2022-09-20
影响因子：
3.4
作者：
Chang, Yanli;Li, Caixia;Liu, Bin
通讯作者：
Liu, Bin
影响因子：
4.2
作者：
Lee, Jungwhoi;Lee, Jungsul;Kim, Jae Hoon
通讯作者：
Kim, Jae Hoon
影响因子：
4.8
作者：
Chen, Jiangyan;Wang, Weitao;Liu, Li
通讯作者：
Liu, Li