Correlation of locomotor activity and brain infarction in rats with transient focal ischemia.
Correlation of locomotor activity and brain infarction in rats with transient focal ischemia.
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DOI：
10.1016/j.jneumeth.2009.11.008
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发表时间：
2010-02-15
影响因子：
3
通讯作者：
Wang, Yun
中科院分区：
文献类型：
--
作者：
Shen, Hui;Wang, Yun
大脑中动脉闭塞(MCAO)是实验动物常用的卒中模型。在这个模型中，不同的行为分析被用来评估损伤的严重程度或治疗后的恢复。在这项研究中，我们研究了成年SD大鼠短暂性大脑中动脉闭塞后梗塞面积和运动活动之间的相关性。用10-O缝线结扎右侧大脑中动脉30~90min。在MCAO后2天，动物被单独放置在三维红外活动室中1h，测量它们的水平、垂直、刻板和旋转活动。用抬高的身体摆动试验检查身体的不对称性。用氯化三苯基四氮唑染色测定脑梗塞体积。研究发现，脑梗塞体积与垂直活动、垂直运动次数、垂直运动时间等垂直运动之间存在显著的相关性。梗塞体积和身体不对称性之间也有显著的相关性。综上所述，我们的数据表明垂直运动活动和身体不对称性是估计MCAO后啮齿动物损伤大小的有用的行为学指标。
Occlusion of middle cerebral artery (MCAo) is commonly used to generate stroke in experimental animals. Different behavioral assays have been used to evaluate the severity of lesions or recovery after treatment in this model. In this study, we examined the correlation between the size of infarction and locomotor activity after transient MCAo in adult Sprague Dawley rats. The right middle cerebral artery was occluded for 30 to 90 -min by ligation with a 10-O suture. At 2 days after MCAo, animals were individually placed in a 3-D infra-red activity chamber for 1 hour to measure their horizontal, vertical, stereotypic and rotational activity. Body asymmetry was examined using an elevated body swing test. Volume of infarction was measured by triphenyltetrazolium chloride staining. We found that there is a significant correlation between the volume of infarction and vertical movement, such as vertical activity, vertical movement number and vertical movement time. There is also a significant correlation between the infarction volume and body asymmetry. In conclusion, our data suggest that vertical locomotor activity and body asymmetry are useful behavioral indices to estimate the size of lesioning in rodents after MCAo.
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