DNA sequencing by CE.
DNA sequencing by CE.
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DOI：
10.1002/elps.200900218
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发表时间：
2009-06
期刊：
影响因子：
2.9
通讯作者：
Guttman, Andras
中科院分区：
文献类型：
--
作者：
Karger, Barry L.;Guttman, Andras
关键词：
Sequencing of human and other genomes has been at the center of interest in the biomedical field over the past several decades and is now leading toward an era of personalized medicine. During this time, DNA sequencing methods have evolved from the labor intensive slab gel electrophoresis, through automated multicapillary electrophoresis systems using fluorophore labeling with multispectral imaging, to the “next generation” technologies of cyclic array, hybridization based, nanopore and single molecule sequencing. Deciphering the genetic blueprint and follow-up confirmatory sequencing of Homo sapiens and other genomes was only possible by the advent of modern sequencing technologies that was a result of step by step advances with a contribution of academics, medical personnel and instrument companies. While next generation sequencing is moving ahead at break-neck speed, the multicapillary electrophoretic systems played an essential role in the sequencing of the Human Genome, the foundation of the field of genomics. In this prospective, we wish to overview the role of capillary electrophoresis in DNA sequencing based in part of several of our articles in this journal.
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DOI：
10.1073/pnas.70.12.3581
发表时间：
1973-01-01
影响因子：
11.1
作者：
GILBERT, W;MAXAM, A
通讯作者：
MAXAM, A
影响因子：
46.9
作者：
Drmanac, S;Kita, D;Drmanac, R
通讯作者：
Drmanac, R
DOI：
10.1016/s0021-9673(00)90574-0
发表时间：
1988-12-23
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
KASPER, TJ;MELERA, M;BROWNLEE, RG
通讯作者：
BROWNLEE, RG
DOI：
10.1016/s0021-9673(01)95485-8
发表时间：
1985-01-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
HJERTEN, S
通讯作者：
HJERTEN, S
影响因子：
7.4
作者：
DROSSMAN, H;LUCKEY, JA;SMITH, LM
通讯作者：
SMITH, LM