Reciprocal interference between Lactobacillus spp. and Gardnerella vaginalis on initial adherence to epithelial cells.
Reciprocal interference between Lactobacillus spp. and Gardnerella vaginalis on initial adherence to epithelial cells.
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DOI：
10.7150/ijms.6304
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发表时间：
2013
影响因子：
3.6
通讯作者：
Cerca N
中科院分区：
文献类型：
--
作者：
Castro J;Henriques A;Machado A;Henriques M;Jefferson KK;Cerca N
细菌性阴道病（BV）是育龄妇女最常见的阴道疾病。人们普遍认为，从正常微植物群到通常与BV相关的植物群的微生物转换的特征在于特定乳杆菌属物种的阴道定殖减少以及G.迷走菌和其他厌氧菌。然而，导致BV发生的事件顺序仍然不清楚，目前还不清楚乳酸杆菌的减少是厌氧菌种群密度增加的原因还是结果。我们的目标是表征两种阴道加德纳菌菌株之间的相互作用，其中一种菌株分离自健康女性（菌株5-1），另一种菌株分离自诊断为BV的女性（菌株101），阴道乳酸杆菌对宫颈上皮细胞的粘附。为了模拟从阴道健康到BV的转变，首先将乳酸杆菌与上皮细胞一起培养，然后将G。引入了迷走神经张力。我们对G.乳酸杆菌对迷走神经的粘附和G.流浪汉我们的研究结果证实，致病性G vagulocarcinoma 101有一个更高的能力，粘附到宫颈上皮细胞比5-1株。有趣的是，菌株101取代了L. crispatus而不是L.而菌株5-1的影响较小，并且对两个物种的影响没有差异。此外，L. Iners实际上增强了菌株101的粘附，但对菌株5-1没有增强。这些结果表明，引起BV的G. vagelis和L.这可能有助于解释以前的报道，即妇女谁是殖民与L。胰岛素更有可能患上BV。
Bacterial vaginosis (BV) is the most common vaginal disorder in women of child-bearing age. It is widely accepted that the microbial switch from normal microflora to the flora commonly associated with BV is characterized by a decrease in vaginal colonization by specific Lactobacillus species together with an increase of G. vaginalis and other anaerobes. However, the order of events leading to the development of BV remains poorly characterized and it is unclear whether the decrease in lactobacilli is a cause or a consequence of the increase in the population density of anaerobes. Our goal was to characterize the interaction between two Gardnerella vaginalis strains, one of which was isolated from a healthy woman (strain 5-1) and the other from a woman diagnosed with BV (strain 101), and vaginal lactobacilli on the adherence to cervical epithelial cells. In order to simulate the transition from vaginal health to BV, the lactobacilli were cultured with the epithelial cells first, and then the G. vaginalis strain was introduced. We quantified the inhibition of G. vaginalis adherence by the lactobacilli and displacement of adherent lactobacilli by G. vaginalis. Our results confirmed that pathogenic G vaginalis 101 had a higher capacity for adhesion to the cervical epithelial cells than strain 5-1. Interestingly, strain 101 displaced L. crispatus but not L. iners whereas strain 5-1 had less of an effect and did not affect the two species differently. Furthermore, L. iners actually enhanced adhesion of strain 101 but not of strain 5-1. These results suggest that BV-causing G. vaginalis and L. iners do not interfere with one another, which may help to explain previous reports that women who are colonized with L. iners are more likely to develop BV.
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影响因子：
4.4
作者：
Harwich, Michael D., Jr.;Alves, Joao M.;Jefferson, Kimberly K.
通讯作者：
Jefferson, Kimberly K.
影响因子：
2.4
作者：
Zarate, G.;Nader-Macias, M. E.
通讯作者：
Nader-Macias, M. E.
影响因子：
4.2
作者：
Verstraelen, Hans;Verhelst, Rita;Vaneechoutte, Mario
通讯作者：
Vaneechoutte, Mario
影响因子：
7.2
作者：
Gutman, RE;Peipert, JF;Blume, J
通讯作者：
Blume, J
DOI：
10.1073/pnas.1000086107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Macklaim, Jean M.;Gloor, Gregory B.;Reid, Gregor
通讯作者：
Reid, Gregor