Minimum Alveolar Concentration of Sevoflurane in Rabbits with Liver Fibrosis
Minimum Alveolar Concentration of Sevoflurane in Rabbits with Liver Fibrosis
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肝纤维化家兔肺泡七氟醚最低浓度
DOI：
10.1213/ane.0b013e31823feca7
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发表时间：
2012-03
影响因子：
5.7
通讯作者：
Tao Zhu, MD
中科院分区：
文献类型：
--
作者：
Yan Yin, MD, Ming Yan, MD,;Tao Zhu, MD
背景：七氟醚广泛应用于外科手术，对肝功能和肝血流均有影响。然而，肝纤维化对七氟烷最低肺泡浓度（MAC）的影响尚不清楚。因此，我们设计了这项研究，以确定七氟醚在兔肝纤维化中的MAC。方法：30只雄性新西兰白色家兔，体重2.5 kg左右，随机分为2组：纤维化组（n = 20）和正常对照组（n = 10）。肝纤维化组用50%四氯化碳灌胃12周，诱导肝纤维化。麻醉前测定血清总蛋白、白蛋白、球蛋白、总胆汁酸、丙氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶、谷氨酰转肽酶、总胆红素、直接胆红素和间接胆红素的浓度。两组中存活动物的麻醉均用七氟烷诱导和维持。采用标准的夹尾技术测定七氟烷在自主呼吸家兔中的MAC。麻醉后处死动物进行肝脏病理检查。研究结果：注射50%四氯化碳12周后，纤维化组20只家兔中有14只存活，对照组10只家兔中有9只存活。纤维化组中所有存活动物均发生中度至重度肝纤维化。纤维化激发后存活的3只家兔因其他疾病或对疼痛刺激无反应而被排除。与对照组相比，纤维化动物的球蛋白、谷氨酰胺转氨酶和谷氨酰转肽酶水平显著升高。然而，白蛋白和碱性磷酸酶水平在纤维化组中显著低于对照组。在七氟烷麻醉期间，两组的平均动脉血压、心率、呼气末CO2和体温均稳定。纤维化组七氟醚的MAC显著低于对照组（3.52% vs 4.10%，P = 0.018）。结论：七氟醚对肝纤维化家兔的MAC明显降低。
BACKGROUND: Sevoflurane is widely used in patients undergoing surgical procedures, which could affect both the liver function and hepatic blood flow. However, the effects of liver fibrosis on minimum alveolar concentration (MAC) of sevoflurane are still unclear. Therefore, we designed this study to determine the MAC of sevoflurane in rabbits with liver fibrosis. METHODS: Thirty male New Zealand white rabbits weighing approximately 2.5 kg were divided randomly into 2 groups: fibrosis (n = 20) and normal control group (n = 10). The rabbits in the fibrosis group were treated with 50% carbon tetrachloride for 12 weeks to induce liver fibrosis. The serum concentration of total protein, albumin, globulin, total bile acids, alanine aminotransferase, aspartame aminotransferase, alkaline phosphatase, &ggr;-glutamyl transpeptidase, total bilirubin, direct bilirubin, and indirect bilirubin were measured before anesthesia. The anesthesia for animals that survived in both groups was induced and maintained with sevoflurane. A standard tail-clamp technique was used to determine the MAC of sevoflurane in spontaneously breathing rabbits. After anesthesia, animals were killed for liver pathologic examination. RESULTS: Twelve weeks after 50% carbon tetrachloride administration, 14 of 20 rabbits survived in the fibrosis group, and 9 of 10 survived in the control group. All surviving animals in the fibrosis group had developed moderate to severe liver fibrosis. Three rabbits that survived after the fibrosis challenge were excluded for other diseases or no response to pain stimulation. The levels of globulin, aspartame aminotransferase, and &ggr;-glutamyl transpeptidase significantly increased in fibrosis animals compared with controls. However, the albumin and alkaline phosphatase levels were significantly lower in the fibrosis group than in the control group. Mean arterial blood pressure, heart rate, end-tidal CO2, and temperature were stable in both groups during sevoflurane anesthesia. The MAC of sevoflurane was significantly less in the fibrosis group than in the control group (3.52% vs 4.10%, P = 0.018). CONCLUSION: The MAC of sevoflurane decreased significantly in rabbits with liver fibrosis.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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