Immunization with EmCRT-Induced Protective Immunity against Echinococcus multilocularis Infection in BALB/c Mice.
Immunization with EmCRT-Induced Protective Immunity against Echinococcus multilocularis Infection in BALB/c Mice.
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使用 EmCRT 诱导的 BALB/c 小鼠针对多房棘球绦虫感染的保护性免疫进行免疫
DOI：
10.3390/tropicalmed7100279
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发表时间：
2022-10-01
影响因子：
2.9
通讯作者：
Zhao L
中科院分区：
文献类型：
--
作者：
Chen L;Cheng Z;Xian S;Zhan B;Xu Z;Yan Y;Chen J;Wang Y;Zhao L
肺泡棘球蚴病（AE）是由多房棘球蚴幼虫期引起的一种严重的寄生虫性人畜共患病。感染过程中获得性免疫抗原的鉴定对棘球蚴感染疫苗的研制具有重要意义。在这里，我们发现多房绦虫钙蛋白（EmCRT）是一种普遍存在的具有Ca2+结合能力的蛋白，可以被感染多房绦虫的小鼠血清识别。天然EmCRT在多房棘球绦虫幼虫表面以及囊泡和原头节（PSCs）分泌产物中表达。从多房棘球蚴囊泡mRNA中克隆了EmCRT的编码DNA，并在大肠杆菌中表达了重组EmCRT蛋白（rEmCRT）。与接受PBS对照的小鼠相比，接受含弗氏佐剂（FA）配制的可溶性rEmCRT免疫的小鼠对多房绦虫PSCs的攻击产生了43.16%的幼虫囊泡重量减轻，PBS对照具有高滴度的IgG， IgG1和IgG2a抗体反应以及高水平的Th1细胞因子（IFN-γ和IL-2）和Th2细胞因子（IL-4， IL-5和IL-10），由脾细胞产生。我们的研究结果表明，EmCRT是由多房棘球绦虫幼虫分泌的免疫优势蛋白，是一种候选疫苗，可诱导接种棘球绦虫感染的小鼠产生部分保护性免疫。
Alveolar echinococcosis (AE) is a severe parasitic zoonosis caused by the larval stage of Echinococcus multilocularis. The identification of the antigens eliciting acquired immunity during infection is important for vaccine development against Echinococcus infection. Here, we identified that E. multilocularis calreticulin (EmCRT), a ubiquitous protein with a Ca2+-binding ability, could be recognized by the sera of mice infected with E. multilocularis. The native EmCRT was expressed on the surface of E. multilocularis larvae as well as in the secreted products of metacestode vesicles and protoscoleces (PSCs). The coding DNA for EmCRT was cloned from the mRNA of the E. multilocularis metacestode vesicles and a recombinant EmCRT protein (rEmCRT) was expressed in E. coli. Mice immunized with soluble rEmCRT formulated with Freund’s adjuvant (FA) produced a 43.16% larval vesicle weight reduction against the challenge of E. multilocularis PSCs compared to those that received the PBS control associated with a high titer of IgG, IgG1 and IgG2a antibody responses as well as high levels of Th1 cytokines (IFN-γ and IL-2) and Th2 cytokines (IL-4, IL-5 and IL-10), produced by splenocytes. Our results suggest that EmCRT is an immunodominant protein secreted by E. multilocularis larvae and a vaccine candidate that induces partial protective immunity in vaccinated mice against Echinococcus infection.
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影响因子：
3.7
作者：
Bi K;Yang J;Wang L;Gu Y;Zhan B;Zhu X
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DOI：
10.13350/j.cjpb.201207
发表时间：
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期刊：
Zhongguo Bingyuan Shengwuxue Zazhi / Journal of Pathogen Biology
影响因子：
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