Endothelial Glycocalyx Morphology in Different Flow Regions of the Aqueous Outflow Pathway of Normal and Laser-Induced Glaucoma Monkey Eyes.
Endothelial Glycocalyx Morphology in Different Flow Regions of the Aqueous Outflow Pathway of Normal and Laser-Induced Glaucoma Monkey Eyes.
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作者：
观察了实验性青光眼和非实验性青光眼猴小梁流出道的糖萼形态。每只猴子沿单眼（n = 6）或双眼（n = 2）约270度的小梁网（TM）进行激光烧伤，直至眼压升高。部分TM未进行激光治疗。未激光眼（n = 6）作为对照。在15 mmHg下灌注去核眼以测量流出设施，灌注荧光素以评估流出模式，灌注固定用于糖萼标记，并进行电子显微镜处理。在非激光区和对照组的高、低流区测量TM、Schlemm管（SC）、收集器通道（CCs）、巩膜内静脉（isv）和巩膜外静脉（esv）中糖萼的覆盖和厚度。与对照组相比，激光治疗的眼睛流出设施减少（p = 0.02）。非激光区和对照组的糖萼厚度从TM到esv均有所增加（p < 0.05）。在非激光区域，糖萼的远端覆盖率普遍更高（p < 0.05）。在激光区域，TM， SC和cc部分至完全消失，isv和esv显示最小的糖萼。青光眼小梁流出道的糖萼是否减少值得进一步研究。
Glycocalyx morphology was examined in the trabecular outflow pathway of monkey eyes with and without experimental glaucoma. Laser burns were administered along ~270 degrees of the trabecular meshwork (TM) of one eye (n = 6) or both eyes (n = 2) of each monkey until intraocular pressure remained elevated. Portions of the TM were not laser-treated. Unlasered eyes (n = 6) served as controls. Enucleated eyes were perfused at 15 mmHg to measure the outflow facility, perfused with fluorescein to evaluate the outflow pattern, perfusion-fixed for glycocalyx labeling, and processed for electron microscopy. Coverage and thickness of the glycocalyx were measured in the TM, Schlemm’s canal (SC), collector channels (CCs), intrascleral veins (ISVs), and episcleral veins (ESVs) in non-lasered regions and high- and low-flow regions of controls. Compared to controls, laser-treated eyes had decreased outflow facility (p = 0.02). Glycocalyx thickness increased from the TM to ESVs in non-lasered regions and controls (p < 0.05). Glycocalyx coverage was generally greater distally in non-lasered regions (p < 0.05). In lasered regions, TM, SC, and CCs were partly to completely obliterated, and ISVs and ESVs displayed minimal glycocalyx. Whether the glycocalyx is decreased in the trabecular outflow pathway of human glaucomatous eyes warrants investigation.
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DOI：
10.2147/opth.s19453
发表时间：
2011
期刊：
Clinical ophthalmology (Auckland, N.Z.)
影响因子：
--
作者：
Balaiya S;Edwards J;Tillis T;Khetpal V;Chalam KV
通讯作者：
Chalam KV
影响因子：
3.4
作者：
BILL, A
通讯作者：
BILL, A
影响因子：
4.4
作者：
Hann, CR;Bahler, CK;Johnson, DH
通讯作者：
Johnson, DH
影响因子：
5.5
作者：
HELPS, EPW;MCDONALD, DA
通讯作者：
MCDONALD, DA
影响因子：
--
作者：
Markiewicz L;Pytel D;Mucha B;Szymanek K;Szaflik J;Szaflik JP;Majsterek I
通讯作者：
Majsterek I