Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence.
Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence.
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DOI：
10.1128/spectrum.00165-23
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发表时间：
2023-06-15
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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甘蔗枯萎病菌是中国地区甘蔗波卡伯恩病的主要病原菌之一。果胶裂解酶(PL)在果胶降解和真菌毒力中起着关键作用，在多种植物的主要细菌和真菌病原菌中都有广泛的研究。然而，只有少数几个pls被进行了功能研究。在本研究中，我们分析了甘蔗果胶裂解酶基因FsPL的功能。FsPL是蔗糖镰刀菌的关键毒力因子，可诱导植物细胞死亡。FsPL还触发了病原菌相关分子模式(PAMP)触发的免疫(PTI)反应，反映在活性氧物种(ROS)的产生、电解质渗漏和愈伤组织积累的增加，以及防御反应基因的上调。此外，我们的研究还发现，FsPL的信号肽是诱导细胞死亡和PTI反应所必需的。病毒诱导的基因沉默表明，FsPL诱导的烟草细胞死亡是由富含亮氨酸重复序列(LRR)受体样激酶BAK1和SOBIR1介导的。因此，FsPL可能不仅是甘蔗赤霉菌的一个关键毒力因子，而且还可能诱导植物的防御反应。这些发现为果胶裂解酶在宿主-病原体相互作用中的功能提供了新的见解。波卡伯恩病是影响中国甘蔗生产的主要病害之一，严重危害甘蔗生产和经济发展。因此，阐明该病的致病机理，为甘蔗抗多氯联苯品系的选育提供理论依据具有重要意义。本研究旨在分析新近发现的甘蔗果胶裂解酶基因FsPL的功能。FsPL是蔗糖镰刀菌诱导植物细胞死亡的关键毒力因子。我们的结果为果胶裂解酶在宿主-病原菌相互作用中的作用提供了新的见解。
Fusarium sacchari is one of the primary pathogens causing Pokkah Boeng disease (PBD) in sugarcane in China. Pectate lyases (PL), which play a critical role in pectin degradation and fungal virulence, have been extensively studied in major bacterial and fungal pathogens of a wide range of plant species. However, only a few PLs have been functionally investigated. In this study, we analyzed the function of the pectate lyase gene, FsPL, from F. sacchari. FsPL is a key virulence factor of F. sacchari and can induce plant cell death. FsPL also triggers the pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) response in Nicotiana benthamiana, as reflected by increases in reactive oxygen species (ROS) production, electrolyte leakage, and callose accumulation, as well as the upregulation of defense response genes. In addition, our study also found that the signal peptide of FsPL was necessary for induced cell death and PTI responses. Virus-induced gene silencing showed that FsPL-induced cell death in Nicotiana benthamiana was mediated by leucine-rich repeat (LRR) receptor-like kinases BAK1 and SOBIR1. Thus, FsPL may not only be a critical virulence factor for F. sacchari but may also induce plant defense responses. These findings provide new insights into the functions of pectate lyase in host-pathogen interactions. IMPORTANCE Pokkah Boeng disease (PBD) is one of the main diseases affecting sugarcane in China, seriously damaging sugarcane production and economic development. Therefore, it is important to clarify the pathogenic mechanisms of this disease and to provide a theoretical basis for the breeding of PBD-resistant sugarcane strains. The present study aimed to analyze the function of FsPL, a recently identified pectate lyase gene from F. sacchari. FsPL is a key virulence factor of F. sacchari that induces plant cell death. Our results provide new insights into the function of pectate lyase in host-pathogen interactions.
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