Endocytic and recycling endosomes modulate cell shape changes and tissue behaviour during morphogenesis in Drosophila.
Endocytic and recycling endosomes modulate cell shape changes and tissue behaviour during morphogenesis in Drosophila.
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DOI：
10.1371/journal.pone.0018729
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发表时间：
2011-04-14
期刊：
影响因子：
3.7
通讯作者：
Martinez Arias A
中科院分区：
文献类型：
--
作者：
Mateus AM;Gorfinkiel N;Schamberg S;Martinez Arias A
在发育过程中，组织变形对于器官的生成和提供生物体的最终形式是必不可少的。这些变形依赖于单个细胞行为的协调，这些行为起源于亚细胞活动的调节。在这里，我们探讨的作用，内吞和回收组织变形发生在果蝇胚胎的背部关闭。在这个过程中，AS收缩，表皮以协调的方式伸长，导致果蝇胚胎背表皮的不连续性关闭。我们使用显性负性形式的Rab5和Rab11来监测在单细胞水平上改变内吞作用和再循环对组织形态发生的影响。我们发现，不同的要求内吞作用（Rab5）和回收（Rab11）在背关闭，而且我们发现，这两个过程中的差异使用在两个组织。内吞作用在AS中需要在顶端收缩期间去除膜，但在表皮中不是必需的。在AS的早期阶段和表皮中细胞伸长需要回收，这表明在这些过程中膜的作用。我们认为，调节内吞和再循环之间的平衡可以调节形态发生过程中的细胞形态和组织变形。
During development tissue deformations are essential for the generation of organs and to provide the final form of an organism. These deformations rely on the coordination of individual cell behaviours which have their origin in the modulation of subcellular activities. Here we explore the role endocytosis and recycling on tissue deformations that occur during dorsal closure of the Drosophila embryo. During this process the AS contracts and the epidermis elongates in a coordinated fashion, leading to the closure of a discontinuity in the dorsal epidermis of the Drosophila embryo. We used dominant negative forms of Rab5 and Rab11 to monitor the impact on tissue morphogenesis of altering endocytosis and recycling at the level of single cells. We found different requirements for endocytosis (Rab5) and recycling (Rab11) in dorsal closure, furthermore we found that the two processes are differentially used in the two tissues. Endocytosis is required in the AS to remove membrane during apical constriction, but is not essential in the epidermis. Recycling is required in the AS at early stages and in the epidermis for cell elongation, suggesting a role in membrane addition during these processes. We propose that the modulation of the balance between endocytosis and recycling can regulate cellular morphology and tissue deformations during morphogenesis.
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影响因子：
4.8
作者：
Debreceni, B;Gao, Y;Zheng, Y
通讯作者：
Zheng, Y
影响因子：
64.8
作者：
Hartwell, LH;Hopfield, JJ;Murray, AW
通讯作者：
Murray, AW
DOI：
10.1083/jcb.200807020
发表时间：
2008-12-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Harris KP;Tepass U
通讯作者：
Tepass U
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影响因子：
3.3
作者：
Giansanti, Maria Grazia;Belloni, Giorgio;Gatti, Maurizio
通讯作者：
Gatti, Maurizio
影响因子：
64.8
作者：
Hakeda-Suzuki, S;Ng, J;Dickson, BJ
通讯作者：
Dickson, BJ