Action of polystyrene nanoparticles of different sizes on lysosomal function and integrity.
Action of polystyrene nanoparticles of different sizes on lysosomal function and integrity.
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DOI：
10.1186/1743-8977-9-26
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发表时间：
2012-07-12
影响因子：
10
通讯作者：
Pieber TR
中科院分区：
文献类型：
--
作者：
Fröhlich E;Meindl C;Roblegg E;Ebner B;Absenger M;Pieber TR
来自环境暴露于纳米颗粒（NPs）的数据表明，长期暴露可能会增加肺、心血管和神经退行性疾病的发病率。也怀疑NP的细胞内积累损害细胞功能。在内体-溶酶体系统中检测到许多类型的NP，并且在重复暴露后，可能发生内体-溶酶体系统的改变。为了鉴定这种作用，我们比较了不同尺寸（20-500 nm）的羧基聚苯乙烯颗粒（CPS）在短（24 h）和长（72 h-96 h）暴露时间后对内皮细胞系EAhy 926的溶酶体的作用。记录CPS的溶酶体定位以及暴露于CPS后的溶酶体pH、溶酶体膜完整性、内体-溶酶体系统的形态和溶酶体酶、组织蛋白酶B和硫酸酯酶的活性。≤100 nm的CPS在4 h后就已与溶酶体高度共定位，24 h后CPS更大。非细胞毒性浓度的颗粒均未引起溶酶体pH值的显著变化或破坏溶酶体膜的完整性。在暴露24小时时，20 nm CPS诱导内体-溶酶体系统的显著扩张和溶酶体硫酸酯酶活性的降低。72小时后，这些变化不太明显。尽管在溶酶体中蓄积，CPS仅诱导溶酶体中的微小变化。接触时间越长，这些变化就越不明显。所提出的一组测定可用于鉴定其他NP对溶酶体的影响。
Data from environmental exposure to nanoparticles (NPs) suggest that chronic exposure may increase the incidence of lung, cardiovascular and neurodegenerative diseases. Impairment of cell function by intracellular accumulation of NPs is also suspected. Many types of NPs have been detected in the endosomal-lysosomal system and, upon repeated exposure, alterations of the endosomal-lysosomal system may occur. To identify such effects we compared the effect of carboxyl polystyrene particles (CPS) of different sizes (20-500 nm) on lysosomes of the endothelial cell line EAhy926 after short (24h) and long (72h-96h) exposure times. Lysosomal localization of CPS, as well as lysosomal pH, lysosomal membrane integrity, morphology of the endosomal-lysosomal system and activities of the lysosomal enzymes,cathepsin B and sulfatases, upon exposure to CPS were recorded. CPS in sizes ≤100 nm showed high co-localization with lysosomes already after 4h, larger CPS after 24h. None of the particles at non-cytotoxic concentrations caused marked changes in lysosomal pH or destroyed lysosomal membrane integrity. At 24h of exposure, 20 nm CPS induced significant dilatation of the endosomal-lysosomal system and reduced activity of lysosomal sulfatases. After 72h, these alterations were less pronounced. Despite accumulation in lysosomes CPS induced only small changes in lysosomes. Upon longer contact, these changes are even less pronounced. The presented panel of assays may serve to identify effects on lysosomes also for other NPs.
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DOI：
10.3402/nano.v1i0.4889
发表时间：
2010
期刊：
Nano reviews
影响因子：
--
作者：
Lévy R;Shaheen U;Cesbron Y;Sée V
通讯作者：
Sée V
影响因子：
5
作者：
Froehlich, Eleonore;Meindl, Claudia;Pieber, Thomas R.
通讯作者：
Pieber, Thomas R.
影响因子：
46.9
作者：
Jaiswal, JK;Mattoussi, H;Simon, SM
通讯作者：
Simon, SM
影响因子：
10.4
作者：
通讯作者：
--
影响因子：
3.9
作者：
Goya, G. F.;Marcos-Campos, I.;Tres, A.
通讯作者：
Tres, A.