The pivotal role of aristaless in development and evolution of diverse antennal morphologies in moths and butterflies.
The pivotal role of aristaless in development and evolution of diverse antennal morphologies in moths and butterflies.
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歧义在飞蛾和蝴蝶中各种触角形态的发展和演变中的关键作用。
DOI：
10.1186/s12862-018-1124-2
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发表时间：
2018-01-25
影响因子：
3.4
通讯作者：
Kojima T
中科院分区：
文献类型：
--
作者：
Ando T;Fujiwara H;Kojima T
嗅器是昆虫的多节附肢，是昆虫的主要嗅觉器官。在鳞翅目昆虫（蛾和蝴蝶）中，触角形态根据其生态要求而多样化。白天活动的蝴蝶有简单的杆状触角，而夜间活动的飞蛾有触角，每个触角节上都有突起或侧枝，用于高灵敏度的信息素检测。以前的一项研究表明，在变态过程中，每节形成两个侧枝的家蚕（蚕蛾）触角的触角图案基因的表达发生了戏剧性的变化，节段重复，分支相关的模式和丰富的细胞增殖，几乎所有的侧分支的背侧。因此，局部细胞增殖可能控制的分支相关的触角图案基因的表达是牵连在横向分支的形成。然而，家蚕侧分支形成的基因功能和鳞翅目昆虫触角形态的进化机制仍不清楚。我们通过电穿孔介导的siRNA或吗啉代寡聚体的掺入，研究了几个基因的功能和信号传导特异性地在家蚕侧分支形成中的作用。敲下aristaless，一个同源异型框基因表达的丰富的细胞增殖的区域内的每个触角节，在变态过程中导致的侧支缺失或大幅缩短，表明其重要性的侧分支的形成。在变态过程中，Distal-less和WNT信号的敲除使无芒表达丧失，而Notch信号的敲除使无芒表达解除，这表明它们的组合作用严格决定了每个触角节内的无芒表达结构域。此外，蛹aristaless表达在触角与各种形态的几个鳞翅目物种的分析表明，aristaless表达模式有一个惊人的相关性与触角的形状，而节段重复的表达模式观察到的触角形态无关。我们的结果表明，无芒功能在B侧分支形成中的意义。mori的结果表明，触角各节在变态过程中无芒表达模式的变化是触角形态的重要决定因素之一。根据这些发现，我们提出了一个机制的发育和进化的鳞翅目触角与各种形态。本文的在线版本（10.1186/s12862-018-1124-2）包含补充材料，可供授权用户使用。
Antennae are multi-segmented appendages and main odor-sensing organs in insects. In Lepidoptera (moths and butterflies), antennal morphologies have diversified according to their ecological requirements. While diurnal butterflies have simple, rod-shaped antennae, nocturnal moths have antennae with protrusions or lateral branches on each antennal segment for high-sensitive pheromone detection. A previous study on the Bombyx mori (silk moth) antenna, forming two lateral branches per segment, during metamorphosis has revealed the dramatic change in expression of antennal patterning genes to segmentally reiterated, branch-associated pattern and abundant proliferation of cells contributing almost all the dorsal half of the lateral branch. Thus, localized cell proliferation possibly controlled by the branch-associated expression of antennal patterning genes is implicated in lateral branch formation. Yet, actual gene function in lateral branch formation in Bombyx mori and evolutionary mechanism of various antennal morphologies in Lepidoptera remain elusive. We investigated the function of several genes and signaling specifically in lateral branch formation in Bombyx mori by the electroporation-mediated incorporation of siRNAs or morpholino oligomers. Knock down of aristaless, a homeobox gene expressed specifically in the region of abundant cell proliferation within each antennal segment, during metamorphosis resulted in missing or substantial shortening of lateral branches, indicating its importance for lateral branch formation. aristaless expression during metamorphosis was lost by knock down of Distal-less and WNT signaling but derepressed by knock down of Notch signaling, suggesting the strict determination of the aristaless expression domain within each antennal segment by the combinatorial action of them. In addition, analyses of pupal aristaless expression in antennae with various morphologies of several lepidopteran species revealed that the aristaless expression pattern has a striking correlation with antennal shapes, whereas the segmentally reiterated expression pattern was observed irrespective of antennal morphologies. Our results presented here indicate the significance of aristaless function in lateral branch formation in B. mori and imply that the diversification in the aristaless expression pattern within each antennal segment during metamorphosis is one of the significant determinants of antennal morphologies. According to these findings, we propose a mechanism underlying development and evolution of lepidopteran antennae with various morphologies. The online version of this article (10.1186/s12862-018-1124-2) contains supplementary material, which is available to authorized users.
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影响因子：
2.7
作者：
Angelini, David R.;Kikuchi, Moto;Jockusch, Elizabeth L.
通讯作者：
Jockusch, Elizabeth L.
影响因子：
64.8
作者：
Campbell, G
通讯作者：
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DOI：
10.1007/s00359-005-0081-6
发表时间：
2006-04-01
影响因子：
2.1
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通讯作者：
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DOI：
10.1098/rspb.2014.0970
发表时间：
2014-08-07
影响因子：
4.7
作者：
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通讯作者：
Breinholt, Jesse W.
影响因子：
64.8
作者：
DIAZBENJUMEA, FJ;COHEN, B;COHEN, SM
通讯作者：
COHEN, SM