High‐density adherent culture of CHO cells using rolled scaffold bioreactor
High‐density adherent culture of CHO cells using rolled scaffold bioreactor
复制标题
使用卷式支架生物反应器对 CHO 细胞进行高密度贴壁培养
DOI：
10.1002/bit.28079
复制
发表时间：
2022
影响因子：
3.8
通讯作者：
Park, Kidong
中科院分区：
文献类型：
--
作者：
YekrangSafakar, Ashkan;Mehrnezhad, Ali;Wu, Tongyao;Park, Kidong
生物制药市场的快速扩张需要更有效和可靠的平台来大规模培养哺乳动物细胞。搅拌罐式生物反应器已广泛用于大规模细胞培养。然而，它需要数月的试验和错误来优化每个细胞系的培养条件。在这篇文章中，我们扩展了我们早期对用于高密度贴壁细胞培养的滚动支架（RS）生物反应器的研究，并报告了两种新的RS实现，它们具有极大的可批量制造性，称为Mesh RS和Fiber RS。CHO‐K1细胞在Mesh‐RS和Fiber‐RS生物反应器中成功扩增，平均生长速率分别为1.09 ± 0.04 1/天和0.95 ± 0.07 1/天，高于类似研究中报告的生长速率。Fiber‐RS生物反应器表现出非常高的细胞密度，为72.8 × 106个细胞/ml。此外，将透析器集成到RS生物反应器中，以去除细胞废物并在不干扰细胞的情况下补充营养。通过单独收集透析介质，透析效率显著提高。总之，所开发的RS生物反应器具有强大的潜力，可为生物制药行业的大规模细胞培养提供高度可靠且易于扩展的平台。
Rapid expansion of biopharmaceutical market calls for more efficient and reliable platforms to culture mammalian cells on a large scale. Stirred‐tank bioreactors have been widely used for large‐scale cell culture. However, it requires months of trials and errors to optimize culture conditions for each cell line. In this article, we extend our earlier studies on rolled scaffold (RS) bioreactors for high‐density adherent cell culture and report two new implementations of RSs with greatly enhanced mass‐manufacturability, termed as Mesh‐RS and Fiber‐RS. CHO‐K1 cells were successfully expanded in Mesh‐RS and Fiber‐RS bioreactors with an average growth rate of 1.09 ± 0.04 1/day and 0.95 ± 0.07 1/day, which were higher than those reported in similar studies. Fiber‐RS bioreactor exhibited a very high cell density of 72.8 × 106cells/ml. Besides, a dialyzer was integrated into the RS bioreactor to remove cellular waste and to replenish nutrients without disturbing the cells. By collecting the dialyzed media separately, the dialysis efficiency was significantly improved. In conclusion, the developed RS bioreactor has a strong potential to provide a highly reliable and easily scalable platform for large‐scale cell culture in the biopharmaceutical industry.
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影响因子：
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作者：
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