Collagen fiber alignment and biaxial mechanical behavior of porcine urinary bladder derived extracellular matrix.
Collagen fiber alignment and biaxial mechanical behavior of porcine urinary bladder derived extracellular matrix.
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DOI：
10.1016/j.biomaterials.2008.08.022
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发表时间：
2008-12
期刊：
影响因子：
14
通讯作者：
Badylak SF
中科院分区：
文献类型：
--
作者：
Gilbert TW;Wognum S;Joyce EM;Freytes DO;Sacks MS;Badylak SF
天然存在的细胞外基质 (ECM) 支架的胶原纤维排列和生物力学行为是用这些材料设计医疗器械的重要考虑因素。为了生产满足组织特定机械要求（例如肌腱与膀胱）的装置，两者都应该被考虑，并且最终可能影响体内的重塑反应。本研究评估了从猪膀胱膜粘膜和基底膜（统称为膀胱基质（UBM））采集的ECM支架材料和从膀胱粘膜下层（UBS）采集的ECM的胶原纤维排列和双轴机械行为。由于 UBM 的制备允许控制分层方向，因此分层方法对 UBM 机械行为的影响是通过沿膀胱纵轴（顶端至颈部）（UBML）或沿圆周方向（UBMC）刮擦粘膜下层和其他近腔层来确定的。 UBS 的处理不允许类似的方向控制。 UBML 和 UBS 具有相似的胶原纤维分布，胶原纤维沿纵向排列优先。 UBMC 显示出更均匀的胶原纤维取向。所有样品在纵向上都表现出更硬的机械行为。尽管胶原纤维分布相似，但 UBML 和 UBS 对于所应用的负载模式表现出截然不同的机械行为，其中 UBS 显示出更明显的脚趾区域。 UBMC 在两个方向上的机械行为与 UBML 的机械行为相似。猪膀胱的不同层 ECM 的机械行为存在明显差异，并且处理方法可以显着改变观察到的机械行为。
The collagen fiber alignment and biomechanical behavior of naturally occurring extracellular matrix (ECM) scaffolds are important considerations for the design of medical devices from these materials. Both should be considered in order to produce a device to meet tissue specific mechanical requirements (e.g., tendon vs. urinary bladder), and could ultimately affect the remodeling response in vivo. The present study evaluated the collagen fiber alignment and biaxial mechanical behavior of ECM scaffold material harvested from porcine urinary bladder tunica mucosa and basement membrane (together referred to as urinary bladder matrix (UBM)) and ECM harvested from urinary bladder submucosa (UBS). Since the preparation of UBM allows for control of the direction of delamination, the effect of the delamination method on the mechanical behavior of UBM was determined by delaminating the submucosa and other abluminal layers by scraping along the longitudinal axis of the bladder (apex to neck) (UBML) or along the circumferential direction (UBMC). The processing of UBS does not allow for similar directional control. UBML and UBS had similar collagen fiber distributions, with a preferred collagen fiber alignment along the longitudinal direction. UBMC showed a more homogenous collagen fiber orientation. All samples showed a stiffer mechanical behavior in the longitudinal direction. Despite similar collagen fiber distributions, UBML and UBS showed quite different mechanical behavior for the applied loading patterns with UBS showing a much more pronounced toe region. The mechanical behavior for UBMC in both directions was similar to the mechanical behavior of UBML. There are distinct differences in the mechanical behavior of different layers of ECM from the porcine urinary bladder, and the processing methods can substantially alter the mechanical behavior observed.
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影响因子：
--
作者：
Gilbert, Thomas W.;Stewart-Akers, Ann M.;Woo, Savio L-Y.
通讯作者：
Woo, Savio L-Y.
影响因子：
14
作者：
Brown, AL;Farhat, W;Woodhouse, KA
通讯作者：
Woodhouse, KA
影响因子：
2.4
作者：
Badylak, S;Meurling, S;Simmons-Byrd, A
通讯作者：
Simmons-Byrd, A
影响因子：
4.9
作者：
Raghavan, D;Kropp, BP;Madihally, SV
通讯作者：
Madihally, SV
DOI：
10.1115/1.2354200
发表时间：
2006-12-01
影响因子：
1.7
作者：
Gilbert, Thomas W.;Sacks, Michael S.;Chancellor, Michael B.
通讯作者：
Chancellor, Michael B.