Monitoring of Serial Presurgical and Postsurgical Changes in the Serum Proteome in a Series of Patients with Calcific Aortic Stenosis.
Monitoring of Serial Presurgical and Postsurgical Changes in the Serum Proteome in a Series of Patients with Calcific Aortic Stenosis.
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DOI：
10.1155/2015/694120
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Matsumoto K
中科院分区：
文献类型：
--
作者：
Satoh K;Yamada K;Maniwa T;Oda T;Matsumoto K
背景对手术或药物治疗后差异表达的蛋白质组进行全面分析有助于了解对分配干预措施的生物学反应。在这里，我们研究了钙化性主动脉瓣狭窄（CAS）患者在主动脉瓣置换术前后血清中表达的变化。材料和方法。用iTRAQ标记随后用nanoLC-MALDI-TOF/TOF-MS/MS分析手术前后获得的具有高丰度蛋白质耗尽的血清。在5例患者中共有51种蛋白质，在术后和术前血清中鉴定出不同水平。最后，鉴定出16种蛋白质，其在患者血清中与对照血清中的那些相比显示统计学显著水平（P < 0.05）。所鉴定的蛋白质多为急性时相蛋白。在急性期蛋白以外的三种蛋白质中，我们使用其他CAS患者的血清通过Western印迹分析证实了术后血清中抗凝血酶-III和锌-α-2-糖蛋白的水平增加。此外，我们在先前的研究中发现，在主动脉瘤患者术后和术前血清中，抗凝血酶-III和锌-α-2-糖蛋白的水平差异蛋白中未发现。结论.结果提示，抗凝血酶-III和锌-α-2-糖蛋白可能成为评价CAS手术前后病理生理生化过程的独特监测蛋白。
Background. Comprehensive analysis of proteome differentially expressed in response to surgery or drug treatment is useful to understand biological responses to dispensed interventions. Here we investigated expression changes in sera of patients who suffered from calcific aortic stenosis (CAS), before and after surgery for aortic valve replacement. Materials and Methods. Sera obtained before and after surgery with depletion of highly abundant proteins were analyzed with iTRAQ labeling followed by nanoLC-MALDI-TOF/TOF-MS/MS. Results. Fifty-one proteins shared in five patients were identified with differential levels in postsurgical and presurgical sera. Finally, 16 proteins that show statistically significant levels in patients' sera compared with those in control sera (P < 0.05) were identified. Most of the identified proteins were positive acute-phase proteins. Among three proteins other than acute-phase proteins, we confirmed increased levels of antithrombin-III and zinc-α-2-glycoprotein in postsurgical sera by Western blot analysis using other CAS patients' sera. Furthermore, antithrombin-III and zinc-α-2-glycoprotein were not found among proteins with differential levels in postsurgical and presurgical sera of patients with aortic aneurysms that we identified in a previous study. Conclusions. The results indicated that antithrombin-III and zinc-α-2-glycoprotein would become unique monitoring proteins for evaluating pathophysiological and biochemical processes occurring before and after surgery for CAS.
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影响因子：
2
作者：
Satoh K;Maniwa T;Oda T;Matsumoto K
通讯作者：
Matsumoto K
DOI：
10.2174/187152508785909537
发表时间：
2008-10-01
影响因子：
--
作者：
Correale, Michele;Brunetti, Natale Daniele;Di Biase, Matteo
通讯作者：
Di Biase, Matteo
DOI：
10.1007/s11936-005-0028-9
发表时间：
2005-12-01
影响因子：
--
作者：
Rajamannan, Nalini M
通讯作者：
Rajamannan, Nalini M
影响因子：
3.5
作者：
CASTELL, JV;GOMEZLECHON, MJ;HEINRICH, PC
通讯作者：
HEINRICH, PC
影响因子：
7
作者：
Ross, PL;Huang, YLN;Pappin, DJ
通讯作者：
Pappin, DJ