Male-derived transcripts isolated from the mated female reproductive tract in Drosophila melanogaster.
Male-derived transcripts isolated from the mated female reproductive tract in Drosophila melanogaster.
复制标题
从果蝇果蝇中配合的女性生殖道中分离出的男性衍生的转录本。
DOI：
10.1093/g3journal/jkad202
复制
发表时间：
2023-11-01
影响因子：
2.6
通讯作者：
Begun, David J.
中科院分区：
文献类型：
--
作者：
Cridland, Julie M.;Begun, David J.
在有内部受精的物种中，精子和精液在交配过程中从雄性转移到雌性。虽然精子和精液都含有包括RNA在内的各种分子，但这些分子中的大多数在受精协调或其他可能的功能中所起的作用还知之甚少。在果蝇中，产生精液的副腺的外体被转移到雌性体内，但它们的潜在货物尚未被描述。此外，虽然在几个哺乳动物物种中已经描述了精子的RNA组成，但在果蝇中对这个问题的研究很少。在这里，我们利用一组高度近交系的雄性和雌性之间的单核苷酸多态差异，以及来自雌性生殖道、精子、睾丸和附腺的转录组数据，来研究从3个雌性生殖器官分离的RNA分子的潜在来源，雄性和雌性，精囊和精囊，其中精囊和精囊储存精子，而卵巢旁腺不储存精子。我们发现交配的雌性携带着来自许多基因的雄性转录本，包括那些作为附腺和已知精液蛋白的标记的基因。我们的观察还支持这样的观点，即可以从女性精子存储器官中分离出完整的精子转录产物。
In species with internal fertilization, sperm, and seminal fluid are transferred from male to female during mating. While both sperm and seminal fluid contain various types of molecules, including RNA, the role of most of these molecules in the coordination of fertilization or in other possible functions is poorly understood. In Drosophila, exosomes from the accessory gland, which produces seminal fluid, are transferred to females, but their potential cargoes have not been described. Moreover, while the RNA composition of sperm has been described in several mammalian species, little work on this problem has occurred in Drosophila. Here we use single nucleotide polymorphism differences between males and females from a set of highly inbred lines of D. melanogaster, and transcriptome data from the female reproductive tract, sperm, testis, and accessory gland, to investigate the potential origin, male vs female, RNA molecules isolated from 3 female reproductive tract organs, the seminal receptacle and spermatheca, which store sperm, and the parovaria, which does not. We find that mated females carry male-derived transcripts from many genes, including those that are markers of the accessory gland and known seminal fluid proteins. Our observations also support the idea that intact sperm transcripts can be isolated from the female sperm storage organs.
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DOI：
10.1073/pnas.92.22.10114
发表时间：
1995-10-24
影响因子：
11.1
作者：
HERNDON, LA;WOLFNER, MF
通讯作者：
WOLFNER, MF
鉴定调节果蝇雄性生殖成功的微肽和多个次级细胞基因。
DOI：
10.1073/pnas.2001897118
发表时间：
2021-04-13
影响因子：
11.1
作者：
Immarigeon C;Frei Y;Delbare SYN;Gligorov D;Machado Almeida P;Grey J;Fabbro L;Nagoshi E;Billeter JC;Wolfner MF;Karch F;Maeda RK
通讯作者：
Maeda RK
影响因子：
3.3
作者：
Gramates LS;Agapite J;Attrill H;Calvi BR;Crosby MA;Dos Santos G;Goodman JL;Goutte-Gattat D;Jenkins VK;Kaufman T;Larkin A;Matthews BB;Millburn G;Strelets VB;the FlyBase Consortium
通讯作者：
the FlyBase Consortium
影响因子：
4.9
作者：
Giardina TJ;Beavis A;Clark AG;Fiumera AC
通讯作者：
Fiumera AC
DOI：
10.1083/jcb.201401072
发表时间：
2014-09-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Corrigan L;Redhai S;Leiblich A;Fan SJ;Perera SM;Patel R;Gandy C;Wainwright SM;Morris JF;Hamdy F;Goberdhan DC;Wilson C
通讯作者：
Wilson C