In vitro culture of Plasmodium berghei-ANKA maintains infectivity of mouse erythrocytes inducing cerebral malaria.
In vitro culture of Plasmodium berghei-ANKA maintains infectivity of mouse erythrocytes inducing cerebral malaria.
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Berghei-Anka疟原虫的体外培养保持了诱导脑疟疾的小鼠红细胞的感染性。
DOI：
10.1186/1475-2875-10-346
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发表时间：
2011-11-25
期刊：
影响因子：
3
通讯作者：
Grau GE
中科院分区：
文献类型：
--
作者：
Jambou R;El-Assaad F;Combes V;Grau GE
伯氏疟原虫感染是一种广泛使用的鼠疟模型，也是研究鼠疟逆转遗传和发病机制的有力工具。然而，红细胞的体外再侵袭的功效通常较低，限制了体外研究。将从易感感染小鼠获得的伯氏疟原虫ANKA感染的血液在各种条件下培养，并每天测量体外寄生虫血症以评估再侵袭率。采用高质量的培养基，并通过剧烈摇瓶和用明胶增加培养基密度来提高再侵染率。使用这些设置，在体外两周内获得寄生虫对正常小鼠红细胞的再侵袭，并保留体内感染性。
Infection with Plasmodium berghei is a widely used model of murine malaria and a powerful tool for reverse genetic and pathogenesis studies. However, the efficacy of in vitro reinvasion of erythrocytes is generally low, limiting in vitro studies. Plasmodium berghei ANKA-infected blood obtained from a susceptible infected mouse was cultured in various conditions and in vitro parasitaemia was measured every day to evaluate the rate of reinvasion. High quality culture media were used and reinvasion rates were improved by vigorous orbital shaking of the flask and increasing density of the medium with gelatin. Using these settings, reinvasion of normal mouse erythrocytes by the parasite was obtained in vitro over two weeks with preservation of the infectivity in vivo.
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DOI：
10.1016/0035-9203(82)90203-6
发表时间：
1982-01-01
影响因子：
2.2
作者：
REST, JR
通讯作者：
REST, JR
影响因子：
1.3
作者：
MCALISTER, RO
通讯作者：
MCALISTER, RO
影响因子：
20.3
作者：
Evans, KJ;Hansen, DS;Schofield, L
通讯作者：
Schofield, L
影响因子：
2.4
作者：
MONS, B;JANSE, CJ;VANDERKAAY, HJ
通讯作者：
VANDERKAAY, HJ
影响因子：
4
作者：
RAMAIYA, ML;KAMATH, VR;RENAPURKAR, DM
通讯作者：
RENAPURKAR, DM