Fine mapping of stable QTLs related to eating quality in rice (Oryza sativa L.) by CSSLs harboring small target chromosomal segments.
Fine mapping of stable QTLs related to eating quality in rice (Oryza sativa L.) by CSSLs harboring small target chromosomal segments.
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DOI：
10.1270/jsbbs.61.338
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发表时间：
2011-12
期刊：
影响因子：
2.4
通讯作者：
Wan J
中科院分区：
文献类型：
--
作者：
Li J;Zhang W;Wu H;Guo T;Liu X;Wan X;Jin J;Hanh TT;Thoa NT;Chen M;Liu S;Chen L;Liu X;Wang J;Zhai H;Wan J
直链淀粉含量和粘度特性是评价稻米食味和蒸煮品质的主要指标。利用染色体片段代换系（CSSLs），在第8染色体上的R727-G1149区间发现了一个控制稻米食味和蒸煮品质的QTL簇。本研究利用快速粘度分析仪（RVA）检测了两个控制回消粘度（SBV）和稠度粘度（CSV）的QTL，它们位于同一区间。以前报道的AC QTL被分解成两个组件具有相反的遗传效应。在4个QTL中，qCSV-8和qAC-8-2分别在3种和4种环境下具有稳定的遗传效应。qSBV-8、qCSV-8和qAC-8-1部分重叠，但与qAC-8-2分离。根据水稻基因芯片的数据，在qAC-8-2位点选择两个与淀粉生物合成相关的基因进行进一步的定量表达分析。这两个基因在携带目标qAC-8-2等位基因的亚CSSLs中表现出增强的表达，但在不含目标qAC-8-2等位基因的亚CSSLs中没有表现出增强的表达，表明它们可能在稻米品质决定中起作用。与这两个稳定QTL紧密连锁的分子标记为优质水稻的分子标记辅助选择提供了可能。
Amylose content (AC) and viscosity profile are primary indices for evaluating eating and cooking qualities of rice grain. Using chromosome segment substitution lines (CSSLs), previous studies identified a QTL cluster of genes for rice eating and cooking quality in the interval R727–G1149 on chromosome 8. In this study we report two QTLs for viscosity parameters, respectively controlling setback viscosity (SBV) and consistency viscosity (CSV), located in the same interval using rapid viscosity analyzer (RVA) profile as an indicator of eating quality. Previously reported QTL for AC was dissected into two components with opposite genetic effects. Of four QTLs, qCSV-8 and qAC-8-2 had stable genetic effects across three and four environments, respectively. qSBV-8, qCSV-8 and qAC-8-1 partly overlapped, but were separated from qAC-8-2. Based on data from an Affymetrix rice GeneChip, two genes related to starch biosynthesis at the qAC-8-2 locus were chosen for further quantitative expression analysis. Both genes showed enhanced expression in sub-CSSLs carrying the target qAC-8-2 allele, but not in sub-CSSLs without the target qAC-8-2 allele, indicating their possible role in rice quality determination. Molecular markers closely linked to the two stable QTL provide the opportunity for marker-assisted selection (MAS) in breeding high quality rice.
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影响因子：
2.3
作者：
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通讯作者：
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影响因子：
4.4
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作者：
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