A secreted ribonuclease effector from Verticillium dahliae localizes in the plant nucleus to modulate host immunity.
A secreted ribonuclease effector from Verticillium dahliae localizes in the plant nucleus to modulate host immunity.
复制标题
DOI：
10.1111/mpp.13213
复制
发表时间：
2022-08
影响因子：
4.9
通讯作者：
Dai, Xiao-Feng
中科院分区：
文献类型：
--
作者：
Yin, Chun-Mei;Li, Jun-Jiao;Wang, Dan;Zhang, Dan-Dan;Song, Jian;Kong, Zhi-Qiang;Wang, Bao-Li;Hu, Xiao-Ping;Klosterman, Steven J.;Subbarao, Krishna, V;Chen, Jie-Yin;Dai, Xiao-Feng
真菌病原体和植物寄主之间的军备竞赛涉及对真菌效应物的识别以诱导寄主免疫。虽然已经发现了各种真菌效应物，但核糖核酸酶的效应功能在很大程度上是未知的。在此，我们发现了一种由大丽花黄萎病（Verticillium dahliae, VdRTX1）分泌的核糖核酸酶，该酶可转运到植物核中调节免疫。VdRTX1的活性可引起烟叶和棉花的超敏反应相关细胞死亡。VdRTX1具有一个信号肽，但不太可能是胞外效应物，因为它在植物中的核定位是诱导细胞死亡所必需的。敲除VdRTX1显著增强了大丽花病菌对烟草的毒力，而大丽花病菌利用已知的抑制因子VdCBM1来逃避VdRTX1诱导的免疫。VdRTX1同源物在真菌中广泛分布，但来自其他真菌的24个同源物的短暂表达并未诱导细胞死亡，这表明该功能是大丽花中VdRTX1所特有的。VdRTX1位点导向突变体在benthamiana叶片中的表达，揭示了VdRTX1在诱导细胞死亡中发挥重要作用的保守配体结合位点。因此，VdRTX1在大丽花中作为一种独特的HR诱导效应物，有助于激活植物免疫。大丽花黄萎病核糖核酸酶VdRTX1是调节植物免疫的效应物。
The arms race between fungal pathogens and plant hosts involves recognition of fungal effectors to induce host immunity. Although various fungal effectors have been identified, the effector functions of ribonucleases are largely unknown. Herein, we identified a ribonuclease secreted by Verticillium dahliae (VdRTX1) that translocates into the plant nucleus to modulate immunity. The activity of VdRTX1 causes hypersensitive response (HR)‐related cell death in Nicotiana benthamiana and cotton. VdRTX1 possesses a signal peptide but is unlikely to be an apoplastic effector because its nuclear localization in the plant is necessary for cell death induction. Knockout of VdRTX1 significantly enhanced V. dahliae virulence on tobacco while V. dahliae employs the known suppressor VdCBM1 to escape the immunity induced by VdRTX1. VdRTX1 homologs are widely distributed in fungi but transient expression of 24 homologs from other fungi did not yield cell death induction, suggesting that this function is specific to the VdRTX1 in V. dahliae. Expression of site‐directed mutants of VdRTX1 in N. benthamiana leaves revealed conserved ligand‐binding sites that are important for VdRTX1 function in inducing cell death. Thus, VdRTX1 functions as a unique HR‐inducing effector in V. dahliae that contributes to the activation of plant immunity. Verticillium dahliae ribonuclease VdRTX1 functions as an effector to modulate plant immunity.
登录
查看更多内容
Wolf Psort：蛋白质定位预测因子。
影响因子：
14.9
作者：
Horton, Paul;Park, Keun-Joon;Obayashi, Takeshi;Fujita, Naoya;Harada, Hajime;Adams-Collier, C J;Nakai, Kenta
通讯作者：
Nakai, Kenta
影响因子：
14.9
作者：
Käll L;Krogh A;Sonnhammer EL
通讯作者：
Sonnhammer EL
影响因子：
56.9
作者：
de Jonge, Ronnie;van Esse, H. Peter;Thomma, Bart P. H. J.
通讯作者：
Thomma, Bart P. H. J.
大丽黄萎病通过糖苷水解酶 12 蛋白与碳水化合物结合模块 1 结合来操纵植物免疫
影响因子：
5.1
作者：
Gui, Yue-Jing;Chen, Jie-Yin;Dai, Xiao-Feng
通讯作者：
Dai, Xiao-Feng
比较基因组学揭示了大丽黄萎病灵活基因组区域中棉花特异性毒力因子以及镰刀菌水平基因转移的证据
DOI：
10.1111/nph.14861
发表时间：
2018-01
期刊：
The New phytologist
影响因子：
--
作者：
Chen JY;Liu C;Gui YJ;Si KW;Zhang DD;Wang J;Short DPG;Huang JQ;Li NY;Liang Y;Zhang WQ;Yang L;Ma XF;Li TG;Zhou L;Wang BL;Bao YM;Subbarao KV;Zhang GY;Dai XF
通讯作者：
Dai XF