Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.
Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.
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DOI:
10.1016/j.vetmic.2018.05.011
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发表时间:
2018-07
影响因子:
3.3
通讯作者:
BRaDP1T consortium
中科院分区:
文献类型:
--
作者:
Bossé JT;Li Y;Fernandez Crespo R;Lacouture S;Gottschalk M;Sárközi R;Fodor L;Casas Amoribieta M;Angen Ø;Nedbalcova K;Holden MTG;Maskell DJ;Tucker AW;Wren BW;Rycroft AN;Langford PR;BRaDP1T consortium
Analysis of complete capsule loci in all 18 serovars of A. pleuropneumoniae. Novel insights into evolution of capsule loci in A. pleuropneumoniae. Development of two mPCRs for comprehensive capsule typing. Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.
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影响因子:
9.4
作者:
Bossé JT;Li Y;Sárközi R;Gottschalk M;Angen Ø;Nedbalcova K;Rycroft AN;Fodor L;Langford PR
通讯作者:
Langford PR
影响因子:
2.4
作者:
Turni, C.;Singh, R.;Blackall, P. J.
通讯作者:
Blackall, P. J.
影响因子:
3.6
作者:
FROSCH, M;EDWARDS, U;WEISGERBER, C
通讯作者:
WEISGERBER, C
影响因子:
3.3
作者:
Angen, Oystein;Ahrens, Peter;Jessing, Stine G.
通讯作者:
Jessing, Stine G.
影响因子:
3.1
作者:
MacLean, LL;Perry, MB;Vinogradov, E
通讯作者:
Vinogradov, E