Repeated administration of a synthetic cannabinoid receptor agonist differentially affects cortical and accumbal neuronal morphology in adolescent and adult rats.
Repeated administration of a synthetic cannabinoid receptor agonist differentially affects cortical and accumbal neuronal morphology in adolescent and adult rats.
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DOI：
10.1007/s00429-014-0914-6
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发表时间：
2016-01
影响因子：
3.1
通讯作者：
Van Bockstaele EJ
中科院分区：
文献类型：
--
作者：
Carvalho AF;Reyes BA;Ramalhosa F;Sousa N;Van Bockstaele EJ
最近的研究表明，在出生后发育过程中，皮质和皮质下大脑区域的大麻素受体表达存在差异轨迹。在本研究中，我们试图调查长期全身暴露于合成大麻素受体激动剂是否会导致青少年和成人内侧前额叶皮层（mPFC）锥体神经元和伏隔核（Acb）中多棘神经元（MSN）树突和树突棘结构的形态学变化。对青少年 (PN 37-40) 和成年 (P55-60) 雄性大鼠全身施用 WIN 55,212-2 后，使用高尔基-考克斯染色评估锥体神经元和 MSN 的神经元结构。虽然与对照相比，在 WIN 55,212-2 治疗的青少年受试者中没有观察到结构变化，但与对照和青少年受试者相比，暴露于 WIN 55,212-2 显着增加了成年受试者 mPFC 中锥体神经元的树突长度、棘密度和树突分支数量。在Acb中，WIN 55,212-2暴露显着降低了成年大鼠受试者的树突长度和分支数量，而在青少年组中没有观察到变化。相比之下，Acb 中成人组和青少年组的脊柱密度均显着降低。为了确定区域发育形态变化是否转化为行为差异，使用条件性位置偏好范式对两组中 WIN 55,212-2 诱导的厌恶进行了评估。在成年大鼠中，WIN 55,212-2 给药很容易诱导条件性地方厌恶，如前所述。相比之下，青春期大鼠在行为范式中接触 WIN 55,212-2 后没有表现出厌恶。目前的结果表明，与青少年受试者相比，合成大麻素的施用对成人的皮质和皮质下神经元形态有不同的影响。这种差异可能是大麻素对行为的不同发育影响的基础。
Recent studies demonstrate a differential trajectory for cannabinoid receptor expression in cortical and sub-cortical brain areas across postnatal development. In the present study, we sought to investigate whether chronic systemic exposure to a synthetic cannabinoid receptor agonist causes morphological changes in the structure of dendrites and dendritic spines in adolescent and adult pyramidal neurons in the medial prefrontal cortex (mPFC) and medium spiny neurons (MSN) in the nucleus accumbens (Acb). Following systemic administration of WIN 55,212-2 in adolescent (PN 37–40) and adult (P55–60) male rats, the neuronal architecture of pyramidal neurons and MSN was assessed using Golgi–Cox staining. While no structural changes were observed in WIN 55,212-2-treated adolescent subjects compared to control, exposure to WIN 55,212-2 significantly increased dendritic length, spine density and the number of dendritic branches in pyramidal neurons in the mPFC of adult subjects when compared to control and adolescent subjects. In the Acb, WIN 55,212-2 exposure significantly decreased dendritic length and number of branches in adult rat subjects while no changes were observed in the adolescent groups. In contrast, spine density was significantly decreased in both the adult and adolescent groups in the Acb. To determine whether regional developmental morphological changes translated into behavioral differences, WIN 55,212-2-induced aversion was evaluated in both groups using a conditioned place preference paradigm. In adult rats, WIN 55,212-2 administration readily induced conditioned place aversion as previously described. In contrast, adolescent rats did not exhibit aversion following WIN 55,212-2 exposure in the behavioral paradigm. The present results show that synthetic cannabinoid administration differentially impacts cortical and sub-cortical neuronal morphology in adult compared to adolescent subjects. Such differences may underlie the disparate development effects of cannabinoids on behavior.
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影响因子：
4.7
作者：
Carlezon WA Jr;Thomas MJ
通讯作者：
Thomas MJ
影响因子：
2.5
作者：
Belleau, ML;Warren, RA
通讯作者：
Warren, RA
影响因子：
10.6
作者：
Davidson, RJ
通讯作者：
Davidson, RJ
影响因子：
3.6
作者：
Bardo, M. T.;Cain, M. E.;Bylica, K. E.
通讯作者：
Bylica, K. E.
影响因子：
5.3
作者：
Baird, Benjamin;Smallwood, Jonathan;Margulies, Daniel S.
通讯作者：
Margulies, Daniel S.