Fiber-optic Raman spectroscopy of joint tissues.
Fiber-optic Raman spectroscopy of joint tissues.
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DOI：
10.1039/c0an00824a
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发表时间：
2011-04-21
期刊：
影响因子：
--
通讯作者：
Roessler BJ
中科院分区：
文献类型：
--
作者：
Esmonde-White KA;Esmonde-White FW;Morris MD;Roessler BJ
在这项研究中，我们报告了使用定制的光纤探头将拉曼光谱应用于关节组织的关节镜检查。在关节镜检查中，区分健康和受损的组织或检查表层下组织(如软骨下骨)是一个挑战，因为肉眼检查可能无法提供足够的对比。通过将点光谱或高光谱成像结合到关节镜检查中，可以改善对健康组织和受损组织的区分，其中对比是基于分子结构或化学成分的。使用定制设计的拉曼光纤探头检查了膝关节身体人体组织和组织模体的关节关节表面。光纤拉曼光谱与用拉曼光谱采集的软骨、软骨下骨和松质骨的参考光谱进行了比较。在关节表面的光纤拉曼光谱中，软骨厚度对软骨下骨信号的恢复有影响。在软骨完整的部位，骨矿化率降低，但对骨矿化学比率的影响很小。制备组织模体作为骨软骨界面的实验模型。组织模体的拉曼光谱表明，软骨的光学散射对软骨和骨的相对信号有很大的影响。骨软骨界面光通量的有限元分析模型证实了在人体身体组织和组织模体中的实验结果。这些最初的研究证明了关节组织的拉曼关节镜测量的原理，并为未来的临床或动物模型研究提供了基础。
In this study, we report adaptation of Raman spectroscopy for arthroscopy of joint tissues using a custom-built fiber optic probe. Differentiation of healthy and damaged tissue or examination of subsurface tissue, such as subchondral bone, is a challenge in arthroscopy because visual inspection may not provide sufficient contrast. Discrimination of healthy versus damaged tissue may be improved by incorporating point spectroscopy or hyperspectral imaging into arthroscopy where contrast is based on molecular structure or chemical composition. Articular joint surfaces of knee cadaveric human tissue and tissue phantoms were examined using a custom-designed Raman fiber optic probe. Fiber-optic Raman spectra were compared against reference spectra of cartilage, subchondral bone and cancellous bone collected using Raman microspectroscopy. In fiber-optic Raman spectra of the articular surface, there was an effect of cartilage thickness on recovery of signal from subchondral bone. At sites with intact cartilage, the bone mineralization ratio decreased but there was a minimal effect in the bone mineral chemistry ratios. Tissue phantoms were prepared as experimental models of the osteochondral interface. Raman spectra of tissue phantoms suggested that optical scattering of cartilage has a large effect on the relative cartilage and bone signal. Finite element analysis modeling of light fluence in the osteochondral interface confirmed experimental findings in human cadaveric tissue and tissue phantoms. These first studies demonstrate proof of principle for Raman arthroscopic measurement of joint tissues and provide a basis for future clinical or animal model studies.
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影响因子：
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