7,8,4'-Trihydroxyisoflavone attenuates DNCB-induced atopic dermatitis-like symptoms in NC/Nga mice.
7,8,4'-Trihydroxyisoflavone attenuates DNCB-induced atopic dermatitis-like symptoms in NC/Nga mice.
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DOI：
10.1371/journal.pone.0104938
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lee KW
中科院分区：
文献类型：
--
作者：
Kim H;Kim JR;Kang H;Choi J;Yang H;Lee P;Kim J;Lee KW
特应性皮炎（AD）是一种以慢性高度炎性和复发性炎症性皮肤病变为特征的疾病。尽管其日益流行，但治疗方法仍然有限。来自草药提取物或衍生物的天然免疫调节剂可用于治疗AD症状。本研究探讨了大豆异黄酮代谢产物7，8，4 ′-三羟基黄酮（7，8，4 ′-THIF）对AD样症状的影响。在NC/Nga小鼠的耳部和背部皮肤上重复表皮应用2，4-二硝基氯苯（DNCB）以诱导AD样症状和皮肤病变，并局部应用7，8，4 ′-THIF（200和400 nmol）或他克莫司（100 µg）3周以评估其抗皮炎作用。我们发现，7，8，4 ′-THIF减轻DNCB诱导的AD样症状，这些症状通过皮肤病变、皮炎评分、耳厚度和抓挠行为来量化。组织学分析表明，7，8，4 ′-THIF减少DNCB诱导的嗜酸性粒细胞和肥大细胞浸润到皮肤病变中。我们还发现，7，8，4 ′-THIF显著减轻DNCB诱导的表皮层水分流失。除了减少DNCB诱导的血清IgE升高外，7，8，4 ′-THIF还降低了趋化因子胸腺和活化调节趋化因子; Th 2细胞因子白细胞介素（IL）-4，IL-5和IL-13;以及Th 1细胞因子IL-12和干扰素-γ的皮肤病变水平。这些结果表明，7，8，4 ′-THIF可能是治疗特应性皮炎的潜在候选药物。
Atopic dermatitis (AD) is characterized by chronic highly pruritic and relapsing inflammatory skin lesions. Despite its growing prevalence, therapeutic treatments remain limited. Natural immune modulators from herbal extracts or derivatives may be useful for treating AD symptoms. This study examined the effect of 7,8,4′-trihydroxyisoflavone (7,8,4′-THIF), a metabolite of soy isoflavone daidzin, on AD-like symptoms. Repeated epicutaneous application of 2,4-dinitrochlorobenzene (DNCB) was performed on the ear and dorsal skin of NC/Nga mice to induce AD-like symptoms and skin lesions, and 7,8,4′-THIF (200 and 400 nmol) or tacrolimus (100 µg) was applied topically for 3 weeks to assess their anti-pruritic effects. We found that 7,8,4′-THIF alleviated DNCB-induced AD-like symptoms as quantified by skin lesion, dermatitis score, ear thickness, and scratching behavior. Histopathological analysis demonstrated that 7,8,4′-THIF decreased DNCB-induced eosinophil and mast cell infiltration into skin lesions. We also found that 7,8,4′-THIF significantly alleviated DNCB-induced loss of water through the epidermal layer. In addition to reducing the DNCB-induced increase in serum IgE, 7,8,4′-THIF also lowered skin lesion levels of the chemokine thymus and activation regulated chemokine; Th2 cytokines interleukin (IL)-4, IL-5, and IL-13; and Th1 cytokines IL-12 and interferon-γ. These results suggest that 7,8,4′-THIF might be a potential therapeutic candidate for the treatment of atopic dermatitis.
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影响因子：
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