Testing the potential of proposed DNA barcoding markers in Nezara virudula and Nezara antennata when geographic variation and closely related species were considered.

Testing the potential of proposed DNA barcoding markers in Nezara virudula and Nezara antennata when geographic variation and closely related species were considered.
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DOI:
10.1093/jis/14.1.79
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发表时间:
2014-05-30
期刊:
Journal of insect science (Online)
影响因子:
--
通讯作者:
Bu W
Bu W
中科院分区:
其他
文献类型:
--
作者:
Li M;Liu Q;Xi L;Liu Y;Zhu G;Zhao Y;Bu W

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COI基因作为动物DNA条形码系统的核心,受到了广泛的关注。观察到的种内和种间序列变异之间的广泛重叠使研究人员设想了主要的基于COI的方法。对稻瘟病菌Nezara virudula(L.)(半翅目:蝽科)的序列,并与N. antennata Scott为分子标记,分析了近缘种的种内和种间关系。综合考虑地理变异和近缘种等因素,Cyt B基因可能是与COI基因并列的候选基因。
The COI gene as the core of the DNA barcoding system for animals has received significant attention. The observed wide overlap between intraand interspecific sequence variability has led researchers to envisage the primary COI -based method. The sequences of 16S rDNA, COI , and Cyt b genes of Nezara virudula (L.) (Hemiptera: Pentatomidae) from 13 countries and the same sequences of N. antennata Scott were chosen as molecular markers to analyze the intraand interspecific relationships between the closely related species in this study. The results support that Cyt b gene may be a good candidate alongside COI, when the combined factors of geographic variation and closely related species are taken into account.
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