Sex-specific gene expression during asexual development of Neurospora crassa.
Sex-specific gene expression during asexual development of Neurospora crassa.
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DOI：
10.1016/j.fgb.2012.05.004
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发表时间：
2012-07
期刊：
影响因子：
--
通讯作者：
Townsend JP
中科院分区：
文献类型：
--
作者：
Wang Z;Kin K;López-Giráldez F;Johannesson H;Townsend JP
基因座的影响，决定性的身份后，无性，占主导地位的阶段真菌生活史已经得到很好的研究。为了研究它们的影响，测定了无性发育期间不同交配型菌株之间的表达差异，其中RNA样品来自于在发育的早期、中期和晚期克隆阶段的粗糙脉孢菌菌株的其他大部分同基因的mat A和mat a菌株。我们观察到在整个克隆发展交配类型之间的整体基因表达的显着差异，特别是在后期发展阶段。通过逆转录和定量PCR分析了交配型基因和信息素基因的表达水平，揭示了两种交配型在各发育阶段的信息素和受体基因的表达，并揭示了交配型（mat）基因在无性发育过程中的表达量逐渐增加。有趣的是，在差异表达的基因中，mat A基因型比mat a基因型更频繁地表现出更高的表达水平，并表现出更大的转录调控动力。在无性发育后期，许多后期光响应基因的表达显著上调。进一步研究光的影响和光反应基因在这两种交配类型的无性发育中的作用是必要的。
The impact of loci that determine sexual identity upon the asexual, dominant stage of fungal life history has been well studied. To investigate their impact, expression differences between strains of different mating type during asexual development were assayed, with RNA sampled from otherwise largely isogenic mat A and mat a strains of Neurospora crassaat early, middle, and late clonal stages of development. We observed significant differences in overall gene expression between mating types across clonal development, especially at late development stages. The expression levels of mating-type genes and pheromone genes were assayed by reverse transcription and quantitative PCR, revealing expression of pheromone and receptor genes instrains of both mating types in all development stages, and revealing that mating type (mat) genes were increasingly expressed over the course of asexual development. Interestingly, among differentially expressed genes, the mat A genotype more frequently exhibited a higher expression level than mat a, and demonstrated greater transcriptional regulatory dynamism. Significant up-regulation of expression was observed for many late light-responsive genes at late asexual development stages. Further investigation of the impact of light and the roles of light response genes in asexual development of both mating types are warranted.
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影响因子：
11.4
作者：
Chen, Chen-Hui;Ringelberg, Carol S.;Loros, Jennifer J.
通讯作者：
Loros, Jennifer J.
DOI：
10.1007/bf00284694
发表时间：
1993-11-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
COPPIN, E;ARNAISE, S;PICARD, M
通讯作者：
PICARD, M
影响因子：
3
作者：
Hallen, Heather E.;Huebner, Marianne;Trail, Frances
通讯作者：
Trail, Frances
粗糙脉孢菌中的长寡聚体微阵列分析揭示了分生孢子萌发的生化和生理事件背后的转录程序。
影响因子：
14.9
作者：
Kasuga, T;Townsend, JP;Tian, CG;Gilbert, LB;Mannhaupt, G;Taylor, JW;Glass, NL
通讯作者：
Glass, NL
影响因子：
3.2
作者：
Funnell, DL;Matthews, PS;VanEtten, HD
通讯作者：
VanEtten, HD