Alternative splicing diversifies the transcriptome and proteome of the rice blast fungus during host infection.
Alternative splicing diversifies the transcriptome and proteome of the rice blast fungus during host infection.
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DOI：
10.1080/15476286.2022.2043040
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发表时间：
2022
期刊：
影响因子：
4.1
通讯作者：
Lee YH
中科院分区：
文献类型：
--
作者：
Jeon J;Kim KT;Choi J;Cheong K;Ko J;Choi G;Lee H;Lee GW;Park SY;Kim S;Kim ST;Min CW;Kang S;Lee YH
选择性剪接(AS)通过从单个基因不同地产生多个mRNA和蛋白质亚型，有助于多样化和调节细胞对环境条件和发育线索的反应。以前对病原真菌中As的研究主要集中在有限条件下的异构体特征。我们利用代表稻瘟病菌营养生长(菌丝)和多个寄主侵染阶段的高质量转录组数据，分析了水稻稻瘟病菌的AS图谱。我们确定了2413个基因的4,270个异构体，其中包括499个可能受感染特异性AS调控的基因。在感染过程中，AS似乎增加，32.7%的AS亚型在感染过程中产生，但在菌丝体中不存在。对每个感染阶段观察到的亚型分析表明，636个AS亚型比相应的注释mRNAs更丰富，尤其是在菌丝最初侵入宿主细胞后。许多这种主要的异构体被预测编码调节蛋白，如转录因子和磷酸转移酶。我们还通过AS鉴定了编码不同蛋白的基因，并通过蛋白质组学分析证实了一些异构体的翻译，表明AS在感染过程中可能介导了一些基因的新功能。水稻不同发育阶段全基因组AS图谱的综合分析。稻瘟病菌互作为研究AS在水稻侵染过程中的作用和调控奠定了基础。
Alternative splicing (AS) contributes to diversifying and regulating cellular responses to environmental conditions and developmental cues by differentially producing multiple mRNA and protein isoforms from a single gene. Previous studies on AS in pathogenic fungi focused on profiling AS isoforms under a limited number of conditions. We analysed AS profiles in the rice blast fungus Magnaporthe oryzae, a global threat to rice production, using high-quality transcriptome data representing its vegetative growth (mycelia) and multiple host infection stages. We identified 4,270 AS isoforms derived from 2,413 genes, including 499 genes presumably regulated by infection-specific AS. AS appears to increase during infection, with 32.7% of the AS isoforms being produced during infection but absent in mycelia. Analysis of the isoforms observed at each infection stage showed that 636 AS isoforms were more abundant than corresponding annotated mRNAs, especially after initial hyphal penetration into host cell. Many such dominant isoforms were predicted to encode regulatory proteins such as transcription factors and phospho-transferases. We also identified the genes encoding distinct proteins via AS and confirmed the translation of some isoforms via a proteomic analysis, suggesting potential AS-mediated neo-functionalization of some genes during infection. Comprehensive profiling of the pattern of genome-wide AS during multiple stages of rice-M. oryzae interaction established a foundational resource that will help investigate the role and regulation of AS during rice infection.
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影响因子：
4.4
作者：
Choi J;Park J;Kim D;Jung K;Kang S;Lee YH
通讯作者：
Lee YH
影响因子：
4.6
作者：
Gonzalez-Hilarion S;Paulet D;Lee KT;Hon CC;Lechat P;Mogensen E;Moyrand F;Proux C;Barboux R;Bussotti G;Hwang J;Coppée JY;Bahn YS;Janbon G
通讯作者：
Janbon G
影响因子：
7.3
作者：
Busch, Anke;Hertel, Klemens J.
通讯作者：
Hertel, Klemens J.
影响因子：
11.6
作者：
Dong, Chunlan;He, Fei;Zheng, Luqing
通讯作者：
Zheng, Luqing
DOI：
10.1242/jeb.088930
发表时间：
2014-01-01
期刊：
The Journal of experimental biology
影响因子：
--
作者：
Brown AJ;Budge S;Kaloriti D;Tillmann A;Jacobsen MD;Yin Z;Ene IV;Bohovych I;Sandai D;Kastora S;Potrykus J;Ballou ER;Childers DS;Shahana S;Leach MD
通讯作者：
Leach MD