Modification of Electrospun Regenerate Cellulose Nanofiber Membrane via Atom Transfer Radical Polymerization (ATRP) Approach as Advanced Carrier for Laccase Immobilization.
Modification of Electrospun Regenerate Cellulose Nanofiber Membrane via Atom Transfer Radical Polymerization (ATRP) Approach as Advanced Carrier for Laccase Immobilization.
复制标题
通过原子转移自由基聚合 (ATRP) 方法对静电纺再生纤维素纳米纤维膜进行改性，作为漆酶固定化的高级载体
DOI：
10.3390/polym13020182
复制
发表时间：
2021-01-06
期刊：
影响因子：
5
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Zeng S;Shi J;Feng A;Wang Z
本研究旨在通过原子转移自由基聚合（ATRP）表面接枝甲基丙烯酸 2-（二甲氨基）乙酯（DMAEMA）作为单体来改性电纺再生纤维素（RC）纳米纤维膜，并研究 ATRP 条件（即引发和聚合）对酶固定化的影响。单体接枝前后纳米纤维膜的各种表征，包括 XPS、FTIR 光谱和 SEM 图像，证实聚 (DMAEMA) 链/刷已成功接枝到 RC 纳米纤维膜上。研究了不同ATRP条件对漆酶固定化的影响，结果表明最佳引发时间和单体接枝时间分别为1和2 h。 RC-Br-1h-poly (DMAEMA)-2h 膜的固定量最高（95.04 ± 4.35 mg），与初始 RC 膜（28.57 ± 3.95 mg）相比增加了约 3.3 倍。所有结果表明，引发和聚合条件的优化是影响酶固定量的关键因素，而ATRP对RC膜进行表面修饰是开发具有高酶负载能力的先进酶载体的一种有前途的方法。
This study aimed to modify an electrospun regenerated cellulose (RC) nanofiber membrane by surface grafting 2-(dimethylamino) ethyl methacrylate (DMAEMA) as a monomer via atom transfer radical polymerization (ATRP), as well as investigate the effects of ATRP conditions (i.e., initiation and polymerization) on enzyme immobilization. Various characterizations including XPS, FTIR spectra, and SEM images of nanofiber membranes before and after monomer grafting verified that poly (DMAEMA) chains/brushes were successfully grafted onto the RC nanofiber membrane. The effect of different ATRP conditions on laccase immobilization was investigated, and the results indicated that the optimal initiation and monomer grafting times were 1 and 2 h, respectively. The highest immobilization amount was obtained from the RC-Br-1h-poly (DMAEMA)-2h membrane (95.04 ± 4.35 mg), which increased by approximately 3.3 times compared to the initial RC membrane (28.57 ± 3.95 mg). All the results suggested that the optimization of initiation and polymerization conditions is a key factor that affects the enzyme immobilization amount, and the surface modification of the RC membrane by ATRP is a promising approach to develop an advanced enzyme carrier with a high enzyme loading capacity.
登录
查看更多内容
影响因子：
5.7
作者：
Mate DM;Alcalde M
通讯作者：
Alcalde M
影响因子：
9.8
作者：
Chang, Renee Han-Yi;Jang, Jen;Wu, Kevin C. -W.
通讯作者：
Wu, Kevin C. -W.
影响因子：
2.5
作者：
El-Aassar, M. R.;Shibraen, Mahmoud H. M. A.;Elzain, Ahmed A.
通讯作者：
Elzain, Ahmed A.
影响因子：
9.8
作者：
Brugnari, Tatiane;Pereira, Marita Gimenez;Peralta, Rosane Marina
通讯作者：
Peralta, Rosane Marina
用于生物医学应用的纤维素纳米晶体和纤维素纳米原纤维基水凝胶
影响因子：
11.2
作者：
Du, Haishun;Liu, Wei;Li, Bin
通讯作者：
Li, Bin