Integration of cell phone imaging with microchip ELISA to detect ovarian cancer HE4 biomarker in urine at the point-of-care.
Integration of cell phone imaging with microchip ELISA to detect ovarian cancer HE4 biomarker in urine at the point-of-care.
复制标题
将手机成像与微芯片ELISA整合在一起，以检测护理点尿液中的卵巢癌HE4生物标志物。
DOI：
10.1039/c1lc20479c
复制
发表时间：
2011-10-21
期刊：
影响因子：
6.1
通讯作者：
Demirci U
中科院分区：
文献类型：
--
作者：
Wang S;Zhao X;Khimji I;Akbas R;Qiu W;Edwards D;Cramer DW;Ye B;Demirci U
卵巢癌在早期阶段是无症状的，大多数患者表现为晚期疾病。缺乏成本效益高的方法，可以实现频繁，简单和非侵入性的测试阻碍了早期发现，并导致卵巢癌患者的高死亡率。在这里，我们报告了一种简单而廉价的基于微芯片ELISA的检测模块，其采用便携式检测系统，即，一个手机/电荷耦合器件（CCD）来量化尿液中的卵巢癌生物标志物HE 4。将移动的应用程序与手机集成，可以立即处理微芯片ELISA结果，从而消除了对笨重、昂贵的分光光度计的需求。通过手机或无透镜CCD系统检测的HE 4水平在来自癌症患者（n = 19）的尿样中比正常健康对照（n = 20）显著升高（p < 0.001）。受试者工作特征（ROC）分析表明，与运行自动分析应用程序的手机耦合的微芯片ELISA具有89.5%的灵敏度和90%的特异性。在相同特异性条件下，微芯片酶联免疫吸附试验的灵敏度为84.2%。总之，微芯片ELISA与基于手机/CCD的比色测量技术的集成可用于在护理点（POC）检测HE 4生物标志物，为创建用于诊断和治疗监测的床边技术铺平了道路。
Ovarian cancer is asymptomatic at early stages and most patients present with advanced levels of disease. Lack of cost-effective methods that can achieve frequent, simple and non-invasive testing hinders early detection and causes high mortality in ovarian cancer patients. Here, we report a simple and inexpensive microchip ELISA-based detection module that employs a portable detection system, i.e., a cell phone/charge-coupled device (CCD) to quantify an ovarian cancer biomarker, HE4, in urine. Integration of a mobile application with a cell phone enabled immediate processing of microchip ELISA results, which eliminated the need for a bulky, expensive spectrophotometer. The HE4 level detected by a cell phone or a lensless CCD system was significantly elevated in urine samples from cancer patients (n = 19) than normal healthy controls (n = 20) (p < 0.001). Receiver operating characteristic (ROC) analyses showed that the microchip ELISA coupled with a cell phone running an automated analysis application had a sensitivity of 89.5% at a specificity of 90%. Under the same specificity, the microchip ELISA coupled with a CCD had a sensitivity of 84.2%. In conclusion, integration of microchip ELISA with cell phone/CCD-based colorimetric measurement technology can be used to detect HE4 biomarker at the point-of-care (POC), paving the way to create bedside technologies for diagnostics and treatment monitoring.
登录
查看更多内容
DOI：
10.1038/nrc2644
发表时间：
2009-06
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
6.1
作者：
Goluch, Edgar D.;Nam, Jwa-Min;Liu, Chang
通讯作者：
Liu, Chang
影响因子：
3.7
作者：
Moon, SangJun;Gurkan, Umut Atakan;Demirci, Utkan
通讯作者：
Demirci, Utkan
影响因子：
3.7
作者：
Granot Y;Ivorra A;Rubinsky B
通讯作者：
Rubinsky B
影响因子：
2.4
作者：
Kakuta, M.;Takahashi, H.;Tokeshi, M.
通讯作者：
Tokeshi, M.