Collective cell guidance by cooperative intercellular forces.
Collective cell guidance by cooperative intercellular forces.
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构成组织的细胞作为集体的一部分迁移。然而，集体过程如何在大型多细胞组装体上协调仍然不清楚，因为在细胞-细胞连接处施加的机械应力尚未通过实验获得。我们在这里报告的地图，这些应力内和细胞之间，包括一个单层。在细胞片层内，会出现意外的机械应力波动，这些波动是严重的，自发出现的，并在整个单层上涟漪。随着系统密度的增加，这种压力环境变得越来越崎岖、缓慢和协作。在这种景观中，局部细胞迁移遵循最大主应力的局部方向。内皮细胞和上皮细胞单层的迁移都符合这种行为，乳腺癌细胞系在上皮-间充质转化之前而不是之后也是如此。在这些不同的系统中，集体迁移被认为是由一个简单但统一的生理学原理所支配的：相邻的细胞联合起来，通过细胞-细胞连接传递可观的正应力，但迁移沿着最小的细胞间剪切应力的方向。
Cells comprising a tissue migrate as part of a collective. How collective processes are coordinated over large multi-cellular assemblies has remained unclear, however, because mechanical stresses exerted at cell-cell junctions have not been accessible experimentally. We report here maps of these stresses within and between cells comprising a monolayer. Within the cell sheet there arise unanticipated fluctuations of mechanical stress that are severe, emerge spontaneously, and ripple across the monolayer. This stress landscape becomes increasingly rugged, sluggish, and cooperative with increasing system density. Within that landscape, local cellular migrations follow local orientations of maximal principal stress. Migrations of both endothelial and epithelial monolayers conform to this behavior, as do breast cancer cell lines before but not after the epithelial-mesenchymal transition. Collective migration in these diverse systems is seen to be governed by a simple but unifying physiological principle: neighboring cells join forces to transmit appreciable normal stress across the cell-cell junction, but migrate along orientations of minimal intercellular shear stress.
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影响因子：
3.8
作者：
Discher, Dennis;Dong, Cheng;Fredberg, Jeffrey J.;Guilak, Farshid;Ingber, Donald;Janmey, Paul;Kamm, Roger D.;Schmid-Schoenbein, Geert W.;Weinbaum, Sheldon
通讯作者：
Weinbaum, Sheldon
DOI：
10.1083/jcb.201001149
发表时间：
2010-06-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
le Duc Q;Shi Q;Blonk I;Sonnenberg A;Wang N;Leckband D;de Rooij J
通讯作者：
de Rooij J
影响因子：
56.9
作者：
Hu, Ke;Ji, Lin;Waterman-Storer, Clare M.
通讯作者：
Waterman-Storer, Clare M.
影响因子：
8.6
作者：
Angelini TE;Hannezo E;Trepat X;Fredberg JJ;Weitz DA
通讯作者：
Weitz DA
DOI：
10.1073/pnas.0914547107
发表时间：
2010-06-01
影响因子：
11.1
作者：
Liu, Zhijun;Tan, John L.;Chen, Christopher S.
通讯作者：
Chen, Christopher S.